Toll-Like Receptor 4 Signaling in the Ileum and Colon of Gnotobiotic Piglets Infected with Salmonella Typhimurium or Its Isogenic Deletion rfa Mutants

0534581 - MBÚ 2021 RIV CH eng J - Journal Article
Šplíchal, Igor - Rychlík, I. - Šplíchalová, Iva - Karasová, D. - Šplíchalová, Alla
Toll-Like Receptor 4 Signaling in the Ileum and Colon of Gnotobiotic Piglets Infected with Salmonella Typhimurium or Its Isogenic Deletion rfa Mutants.
Toxins. Roč. 12, č. 9 (2020), č. článku 545. ISSN 2072-6651. E-ISSN 2072-6651
R&D Projects: GA MŠMT(CZ) LD15090
Institutional support: RVO:61388971 ; RVO:68378050
Keywords : lipopolysaccharide * chemotype * endotoxin * toll-like receptor 4
OECD category: Toxicology; Toxicology (UMG-J)
Impact factor: 4.546, year: 2020
Method of publishing: Open access
https://www.mdpi.com/2072-6651/12/9/545

Salmonella Typhimurium is a Gram-negative bacterium that causes enterocolitis in humans and pigs. Lipopolysaccharide (LPS) is a component of the outer leaflet of Gram-negative bacteria that provokes endotoxin shock. LPS can be synthesized completely or incompletely and creates S (smooth) or R (rough) chemotypes. Toll-like receptors (TLR) 2, 4, and 9 initiate an inflammatory reaction to combat bacterial infections. We associated/challenged one-week-old gnotobiotic piglets with wild-type S. Typhimurium with S chemotype or its isogenic increment rfa mutants with R chemotype LPS. The wild-type S. Typhimurium induced TLR2 and TLR4 mRNA expression but not TLR9 mRNA expression in the ileum and colon of one-week-old gnotobiotic piglets 24 h after challenge. The TLR2 and TLR4 stimulatory effects of the S. Typhimurium increment rfa mutants were related to the completeness of their LPS chain. The transcription of IL-12/23 p40, IFN-gamma, and IL-6 in the intestine and the intestinal and plasmatic levels of IL-12/23 p40 and IL-6 but not IFN-gamma were related to the activation of TLR2 and TLR4 signaling pathways. The avirulent S. Typhimurium increment rfa mutants are potentially useful for modulation of the TLR2 and TLR4 signaling pathways to protect the immunocompromised gnotobiotic piglets against subsequent infection with the virulent S. Typhimurium.
Permanent Link: http://hdl.handle.net/11104/0312753