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Phylogenetic evidence for the invasion of a commercialized European Phasmarhabditis hermaphrodita lineage into North America and New Zealand
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SYSNO ASEP 0531891 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Phylogenetic evidence for the invasion of a commercialized European Phasmarhabditis hermaphrodita lineage into North America and New Zealand Author(s) Howe, D. K. (US)
Ha, A. D. (US)
Colton, A. (US)
Tandingan De Ley, I. (US)
Rae, R. G. (GB)
Ross, J. (ZA)
Wilson, M. (NZ)
Nermuť, Jiří (BC-A) RID, ORCID
Zhao, Z. (CN)
Mc Donnell, R. J. (US)
Denver, D. R. (US)Number of authors 11 Article number e0237249 Source Title PLoS ONE. - : Public Library of Science - ISSN 1932-6203
Roč. 15, č. 8 (2020)Number of pages 14 s. Language eng - English Country US - United States Keywords slug-parasitic nematode ; small Indian mongoose ; Caenorhabditis elegans Subject RIV EG - Zoology OECD category Zoology Method of publishing Open access Institutional support BC-A - RVO:60077344 UT WOS 000563517800018 EID SCOPUS 85089556245 DOI 10.1371/journal.pone.0237249 Annotation Biological control (biocontrol) as a component of pest management strategies reduces reliance on synthetic chemicals, and seemingly offers a natural approach that minimizes environmental impact. However, introducing a new organism to new environments as a classical biocontrol agent can have broad and unanticipated biodiversity effects and conservation consequences. Nematodes are currently used in a variety of commercial biocontrol applications, including the use of Phasmarhabditis hermaphrodita as an agent targeting pest slug and snail species. This species was originally discovered in Germany, and is generally thought to have European origins. P. hermaphrodita is sold under the trade name Nemaslug®, and is available only in European markets. However, this nematode species was discovered in New Zealand and the western United States, though its specific origins remained unclear. In this study, we analyzed 45 nematode strains representing eight different Phasmarhabditis species, collected from nine countries around the world. A segment of nematode mitochondrial DNA (mtDNA) was sequenced and subjected to phylogenetic analyses. Our mtDNA phylogenies were overall consistent with previous analyses based on nuclear ribosomal RNA (rRNA) loci. The recently discovered P. hermaphrodita strains in New Zealand and the United States had mtDNA haplotypes nearly identical to that of Nemaslug®, and these were placed together in an intraspecific monophyletic clade with high support in maximum likelihood and Bayesian analyses. We also examined bacteria that co-cultured with the nematode strains isolated in Oregon, USA, by analyzing 16S rRNA sequences. Eight different bacterial genera were found to associate with these nematodes, though Moraxella osloensis, the bacteria species used in the Nemaslug® formulation, was not detected. This study provided evidence that nematodes deriving from the Nemaslug® biocontrol product have invaded countries where its use is prohibited by regulatory agencies and not commercially available. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2021 Electronic address https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0237249&type=printable
Number of the records: 1