Analysis of major bile acids in saliva samples of patients with Barrett's esophagus using high-performance liquid chromatography-electrospray ionization-mass spectrometry

0531800 - ÚIACH 2021 RIV NL eng J - Journal Article
Ďurč, P. - Dosedělová, V. - Foret, František - Dolina, J. - Konečný, Š. - Himmelsbach, M. - Buchberger, W. - Kubáň, Petr
Analysis of major bile acids in saliva samples of patients with Barrett's esophagus using high-performance liquid chromatography-electrospray ionization-mass spectrometry.
Journal of Chromatography A. Roč. 1625, AUG (2020), s. 1-9, č. článku 461278. ISSN 0021-9673. E-ISSN 1873-3778
Institutional support: RVO:68081715
Keywords : Bile acids * hplc-ms * Saliva
OECD category: Analytical chemistry
Impact factor: 4.759, year: 2020
Method of publishing: Limited access

A fast, non-invasive, high-performance liquid chromatographic screening method with electrospray ionization mass spectrometric detection was developed for the analysis of three major glycine-conjugated bile acids in human saliva. Using a mobile phase composed of 80% methanol and 0.1% formic acid, glycocholic, glycodeoxycholic, and glycochenodeoxycholic acids were separated in less than 4 minutes with sensitivity in the low nM range. Bile acids are thought to contribute to the pathology of various complications in gastroesophageal reflux disease, for instance, Barrett's esophagus, which may eventually lead to esophageal carcinoma. In this pilot study, samples of saliva obtained from 15 patients with Barrett's esophagus of various severities were compared to saliva samples from 10 healthy volunteers. Glycochenodeoxycholic acid was significantly elevated in the patients and principal component analysis of all bile acids could distinguish the most severe Barrett's esophagus patients. We also reported on the detection of glycochenodeoxycholic acid in exhaled breath condensate for the first time. The promising results of this pilot study warrant future investigation, aiming at non-invasive diagnostics of Barrett's esophagus susceptibility in patients with gastroesophageal reflux disease. (C) 2020 Elsevier B.V. All rights reserved.
Permanent Link: http://hdl.handle.net/11104/0310416