Schistosoma mansoni: Methods and Protocols

0525102 - ÚOCHB 2021 RIV US eng M - Monography Chapter
Leontovyč, Adrian - Ulrychová, Lenka - Horn, Martin - Dvořák, Jan
Collection of Excretory/Secretory Products from Individual Developmental Stages of the Blood Fluke Schistosoma mansoni.
Schistosoma mansoni: Methods and Protocols. New York: Humana Press, 2020 - (Timson, D.), s. 55-63. Methods in Molecular Biology, 2151. ISBN 978-1-0716-0634-6
R&D Projects: GA ČR(CZ) GA19-17269S
Institutional support: RVO:61388963
Keywords : Schistosoma mansoni * developmental stages * excretory/secretory products
OECD category: Biochemistry and molecular biology

Individual developmental stages of blood fluke Schistosoma mansoni excrete or secrete a different set of molecules. Here we describe optimized protocols for collection of excretory/secretory products (E/S products) from cercariae, schistosomula, adult worms, and eggs. These E/S products are essential for successful parasitism functioning at the host-parasite interface, enabling invasion into the host and contributing to the survival of the parasite by modulation of host physiology and immune responses. Collection of sufficient amounts of E/S products is required for detailed research of these processes.
Permanent Link: http://hdl.handle.net/11104/0309314