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Chronic exposure of soybean plants to nanomolar cadmium reveals specific additional high-affinity targets of cadmium toxicity
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SYSNO ASEP 0524404 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Chronic exposure of soybean plants to nanomolar cadmium reveals specific additional high-affinity targets of cadmium toxicity Author(s) Andresen, Elisa (BC-A) RID, ORCID
Lyubenova, Lyudmila (BC-A) RID
Hubáček, Tomáš (BC-A) RID
Bokhari, Syed Nadeem Hussain (BC-A) RID
Matoušková, Šárka (GLU-S) RID, SAI
Mijovilovich, Ana (BC-A) ORCID, RID
Rohovec, Jan (GLU-S) RID, SAI
Küpper, Hendrik (BC-A) RID, ORCIDNumber of authors 8 Source Title Journal of Experimental Botany. - : Oxford University Press - ISSN 0022-0957
Roč. 71, č. 4 (2020), s. 1628-1644Number of pages 17 s. Publication form Print - P Language eng - English Country GB - United Kingdom Keywords cadmium ; lipidomics ; metabolomics ; metalloproteomics ; metal stress ; soybean (Glycine max) ; sublethal toxicity ; XANES Subject RIV BO - Biophysics OECD category Biophysics Subject RIV - cooperation Institute of Geology - Biophysics R&D Projects EF15_003/0000336 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) EF16_013/0001782 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LM2015075 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support BC-A - RVO:60077344 ; GLU-S - RVO:67985831 UT WOS 000518530400036 EID SCOPUS 85080844410 DOI 10.1093/jxb/erz530 Annotation Solving the global environmental and agricultural problem of chronic low-level cadmium (Cd) exposure requires better mechanistic understanding. Here, soybean (Glycine max) plants were exposed to Cd concentrations ranging from 0.5 nM (background concentration, control) to 3 mu M. Plants were cultivated hydroponically under non-nodulating conditions for 10 weeks. Toxicity symptoms, net photosynthetic oxygen production and photosynthesis biophysics (chlorophyll fluorescence: Kautsky and OJIP) were measured in young mature leaves. Cd binding to proteins [metalloproteomics by HPLC-inductively coupled plasma (ICP)-MS] and Cd ligands in light-harvesting complex II (LHCII) [X-ray absorption near edge structure (XANES)], and accumulation of elements, chloropyll, and metabolites were determined in leaves after harvest. A distinct threshold concentration of toxicity onset (140 nM) was apparent in strongly decreased growth, the switch-like pattern for nutrient uptake and metal accumulation, and photosynthetic fluorescence parameters such as Phi(RE10) (OJIP) and saturation of the net photosynthetic oxygen release rate. XANES analyses of isolated LHCII revealed that Cd was bound to nitrogen or oxygen (and not sulfur) atoms. Nutrient deficiencies caused by inhibited uptake could be due to transporter blockage by Cd ions. The changes in specific fluorescence kinetic parameters indicate electrons not being transferred from PSII to PSI. Inhibition of photosynthesis combined with inhibition of root function could explain why amino acid and carbohydrate metabolism decreased in favour of molecules involved in Cd stress tolerance (e.g. antioxidative system and detoxifying ligands). Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2021 Electronic address https://academic.oup.com/jxb/article/71/4/1628/5639660
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