Distinguishing the glycan isomers 2,3-sialyllactose and 2,6-sialyllactose by voltammetry after modification with osmium(VI) complexes

Trefulka, Mojmír; Černocká, Hana; Fojt, Lukáš; Paleček, Emil; Ostatná, Veronika

doi:https://dx.doi.org/10.1016/j.aca.2019.03.060

Number of the records: 1

Distinguishing the glycan isomers 2,3-sialyllactose and 2,6-sialyllactose by voltammetry after modification with osmium(VI) complexes

1.

SYSNO ASEP	0507731
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Distinguishing the glycan isomers 2,3-sialyllactose and 2,6-sialyllactose by voltammetry after modification with osmium(VI) complexes
Author(s)	Trefulka, Mojmír (BFU-R)_{RID, ORCID} Černocká, Hana (BFU-R)_{RID, ORCID} Fojt, Lukáš (BFU-R)_{RID, ORCID} Paleček, Emil (BFU-R)_{RID, ORCID} Ostatná, Veronika (BFU-R)_{RID, ORCID}
Number of authors	5
Source Title	Analytica Chimica Acta. - : Elsevier - ISSN 0003-2670 Roč. 1067, AUG 27 2019 (2019), s. 56-62
Number of pages	7 s.
Publication form	Print - P
Language	eng - English
Country	NL - Netherlands
Keywords	glycosylation ; strategies ; proteins ; oligosaccharides ; polysaccharides
Subject RIV	CB - Analytical Chemistry, Separation
OECD category	Analytical chemistry
R&D Projects	GA18-18154S GA ČR - Czech Science Foundation (CSF)
Method of publishing	Limited access
Institutional support	BFU-R - RVO:68081707
UT WOS	000466150300005
DOI	10.1016/j.aca.2019.03.060
Annotation	Altered glycosylation is a universal feature of cancer cells and certain glycans are well-known markers of tumor progression. In this work we studied two glycan isomers, 2,3-sialyllactose (3-SL) and 2,6-sialyllactose (6-SL), frequently appearing in glycoproteins connected with cancer. A combination of square wave voltammetry and glycan modification with osmium(VI) N, N, N', N'-tetramethylethylenedi-amine (Os(VI) tem) allowed to distinguish between these regioisomers, since the 6-SL molecule can bind three Os(VI), while the 3-SL only two Os(VI) moieties, as experiments using capillary electrophoresis, inductively coupled plasma mass spectrometry and thin layer chromatography showed. A similar pattern of Os(VI)-modification was found for isomers of sialyl-N-acetyllactosamine and sialylgalactose. Covalent adducts of Os(VI) tem with glycans yielded three reduction voltammetric peaks. The ratio of peak I/peak II heights depends on the content of individual regioisomer in the sample. Our proposed approach allows the determination of isomer percentage representation in the mixture after one voltammogram recording. These results show a new appropriate method for the discrimination of glycan isomers containing terminal sialic acid important for distinguishing between cancerous and non-cancerous origin of biomarkers. (C) 2019 Elsevier B.V. All rights reserved.
Workplace	Institute of Biophysics
Contact	Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244
Year of Publishing	2020
Electronic address	https://www.sciencedirect.com/science/article/pii/S0003267019303782?via%3Dihub

Number of the records: 1