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Chemiluminescence: A Sensitive Method for Detecting the Effects of Histamine Receptor Agonists/Antagonists on Neutrophil Oxidative Burst
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SYSNO ASEP 0506827 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Chemiluminescence: A Sensitive Method for Detecting the Effects of Histamine Receptor Agonists/Antagonists on Neutrophil Oxidative Burst Author(s) Vašíček, Ondřej (BFU-R) ORCID, RID
Perečko, Tomáš (BFU-R) RID, ORCID
Jančinová, V. (SK)
Pažoureková, S. (SK)
Nosál, R. (SK)
Číž, Milan (BFU-R) RID, ORCIDNumber of authors 6 Source Title Histamine Receptors as Drug Targets - ISSN 1557-2153
-, č. 2017 (2017), s. 183-208Series Methods in Pharmacology and Toxicology Number of pages 26 s. Publication form Print - P Language eng - English Country US - United States Keywords intracellular oxidant production ; dependent chemi-luminescence ; polymorphonuclear leukocytes ; professional phagocytes Subject RIV CE - Biochemistry OECD category Biochemistry and molecular biology Method of publishing Limited access Institutional support BFU-R - RVO:68081707 UT WOS 000412310500008 DOI 10.1007/978-1-4939-6843-5_7 Annotation Polymorphonuclear leukocytes (neutrophils) form the first line of defence in humans. After activation, they migrate through vessel walls toward the invading agent. The intruding pathogens are eliminated through the action of released proteases and by production of reactive oxygen species (ROS). Histamine, a biogenic amine with various physiological and pathological effects, is known to modulate the immune response of neutrophils, e.g., it blunts the production of ROS in neutrophils after binding to the histamine-2 receptor. To detect the ROS in neutrophils, various methods are used. Among these, luminophore-enhanced chemiluminescence is a very sensitive and easy tool for studying the effects of various compounds on ROS production in neutrophils. On the other hand, its selectivity is questionable. However, employing cell-free systems producing selective ROS types, the inhibitory effects of tested compounds against distinct ROS may be evaluated. In this chapter, we describe the methods for the evaluation of ROS modulating effects of histamine agonists/antagonists in human neutrophils by using chemiluminescence methods. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2020 Electronic address https://www.researchgate.net/publication/314265979_Chemiluminescence_A_Sensitive_Method_for_Detecting_the_Effects_of_Histamine_Receptor_AgonistsAntagonists_on_Neutrophil_Oxidative_Burst
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