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lncRNAs in non-malignant tissue have prognostic value in colorectal cancer
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SYSNO ASEP 0495541 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title lncRNAs in non-malignant tissue have prognostic value in colorectal cancer Author(s) Thiele, J. A. (CZ)
Hošek, P. (CZ)
Královcová, E. (CZ)
Ostašov, P. (CZ)
Liška, V. (CZ)
Bruha, J. (CZ)
Vyčítal, O. (CZ)
Rosendorf, J. (CZ)
Opattová, Alena (UEM-P)
Horák, Josef (UEM-P) ORCID
Králíčková, M. (CZ)
Vodička, Pavel (UEM-P) RID
Pitule, P. (CZ)Article number 2672 Source Title International Journal of Molecular Sciences. - : MDPI
Roč. 19, č. 9 (2018)Number of pages 15 s. Language eng - English Country CH - Switzerland Keywords colorectal carcinoma ; lncRNA ; lncRNA ratio ; MIR155HG Subject RIV EB - Genetics ; Molecular Biology OECD category Gastroenterology and hepatology Institutional support UEM-P - RVO:68378041 UT WOS 000449988100208 EID SCOPUS 85053081079 DOI 10.3390/ijms19092672 Annotation Although colorectal cancer-CRC is the third most frequent cause of cancer related death in Europe, biomarkers for therapy guidance and prognosis are insufficiently reliable. Long non-coding RNAs are over 200 nucleotides long, they are not translated into proteins but can influence biological processes, as oncogenes, tumour suppressors or regulators of cell proliferation and metastasis development. We aimed to evaluate the prognostic effect of selected lncRNAs in a retrospective study on CRC patients from the Czech Republic. We have measured the expression in paired non-malignant and tumour tissues of CRC patients of nine lncRNAs previously shown to be involved in cancer progression-ANRIL, CCAT1, GAS5, linc-ROR, MALAT1, MIR155HG, PCAT1, SPRY4-IT1 and TUG1. A comparison of expression in tumour and non-malignant mucosa tissues showed significant upregulation of CCAT1 and linc-ROR in tumor tissue and downregulation of ANRIL, MIR155HG and MALAT1. Linc-ROR was significantly associated with the presence of synchronous metastases. For individual tissue types, lower MIR155HG expression in tumor tissue was correlated with both shorter overall survival and shorter disease-free survival. In non malignant tissue expression ratios of CCAT1/ANRIL and CCAT1/MIR155HG were significantly associated with overall survival. Our results revealed that changes in expression of lncRNAs between tumor and non-malignant issues could be used as prognostic biomarkers in CRC patients. Moreover, the ratios of CCAT1 to ANRIL and MIR155HG in non-malignant issue could be used for prognosis assessment. It seems that cancer progression is associated with detrimental system-wide changes in patient tissue, which might govern patient survival even after elimination of tumour. Workplace Institute of Experimental Medicine Contact Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Year of Publishing 2019
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