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TATAA courses – 2 days Hands-on qPCR

SYSNO ASEP	0486879
Document Type	U - Organizing Conference, Workshop, Exhibition
R&D Document Type	W - Uspořádání workshopu
R&D Document Type	Není vybrán druh dokumentu
Title	TATAA courses – 2 days Hands-on qPCR
Author(s)	Kubista, Mikael (BTO-N)_RID Valihrach, Lukáš (BTO-N)_{RID, ORCID} Tománková, Silvie (BTO-N)_RID Androvič, Peter (BTO-N) Rohlová, Eva (BTO-N) Švec, David (BTO-N)_RID
Event type	W - Workshop
Event date	29.05.2017 - 30.05.2017
VEvent location	Vestec, BTÚ
Country	CZ - Czech Republic
Event type	WRD
Total number of participants	6
Number of foreign participants	4
Language	eng - English
Keywords	qPCR ; biostatistic ; reverese transcription
Subject RIV	EB - Genetics ; Molecular Biology
OECD category	Genetics and heredity (medical genetics to be 3)
R&D Projects	ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	BTO-N - RVO:86652036
Annotation	Day 1 - This introductory course consists of a theoretical part and a practical part where participants get to do qPCR experiments by themselves under experienced supervision. The course contains: Basic PCR theory. The theory of real-time PCR. Applications and possibilities of qPCR. Comparison of qPCR with regular PCR. Review of currently available detection technologies (SYBR Green I, hydrolysis probes, Molecular Beacons...etc). Different instrument platforms and their typical uses. Primer design. The problem of primer-dimer formation and how to minimize them. Probe design. Experimental design and optimization. Basic data handling and analysis. Day 2 - Introduction to quantification principles. Quantification strategies, uses and limitations. Calculations using different relative quantification methods. Strategies for normalization of qPCR data. In situ calibration for compensation of inhibition in samples. Absolute quantification.
Workplace	Institute of Biotechnology
Contact	Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700
Year of Publishing	2018

Number of the records: 1