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Biological characterization of a novel hybrid copolymer carrier system based on glycogen
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SYSNO ASEP 0484217 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Biological characterization of a novel hybrid copolymer carrier system based on glycogen Author(s) Jirátová, M. (CZ)
Pospíšilová, Aneta (UMCH-V)
Rabyk, Mariia (UMCH-V) RID, ORCID
Pařízek, Martin (FGU-C) RID
Kovář, J. (CZ)
Gálisová, A. (CZ)
Hrubý, Martin (UMCH-V) RID, ORCID
Jirák, D. (CZ)Source Title Drug Delivery and Translational Research - ISSN 2190-393X
Roč. 8, č. 1 (2018), s. 73-82Number of pages 10 s. Language eng - English Country DE - Germany Keywords glycogen ; polymers ; drug delivery Subject RIV FR - Pharmacology ; Medidal Chemistry OECD category Pharmacology and pharmacy Subject RIV - cooperation Institute of Physiology - Pharmacology ; Medidal Chemistry R&D Projects NV15-25781A GA MZd - Ministry of Health (MZ) GA13-08336S GA ČR - Czech Science Foundation (CSF) GAP108/12/1168 GA ČR - Czech Science Foundation (CSF) Institutional support UMCH-V - RVO:61389013 ; FGU-C - RVO:67985823 UT WOS 000428713100008 EID SCOPUS 85040062084 DOI 10.1007/s13346-017-0436-x Annotation he effective drug delivery systems for cancer treatment are currently on high demand. In this paper, biological behavior of the novel hybrid copolymers based on polysaccharide glycogen were characterized. The copolymers were modified by fluorescent dyes for flow cytometry, confocal microscopy, and in vivo fluorescence imaging. Moreover, the effect of oxazoline grafts on degradation rate was examined. Intracellular localization, cytotoxicity, and internalization route of the modified copolymers were examined on HepG2 cell line. Biodistribution of copolymers was addressed by in vivo fluorescence imaging in C57BL/6 mice. Our results indicate biocompatibility, biodegradability, and non-toxicity of the glycogen-based hybrid copolymers. Copolymers were endocyted into the cytoplasm, most probably via caveolae-mediated endocytosis. Higher content of oxazoline in polymers slowed down cellular uptake. No strong colocalization of the glycogen-based probe with lysosomes was observed. Thus, it seems that the modified externally administered glycogen is degraded in the same way as an endogenous glycogen. In vivo experiment showed relatively fast biodistribution and biodegradation. In conclusion, this novel nanoprobe offers unique chemical and biological attributes for its use as a novel drug delivery system that might serve as an efficient carrier for cancer therapeutics with multimodal imaging properties. Workplace Institute of Macromolecular Chemistry Contact Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Year of Publishing 2019
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