Number of the records: 1  

In vivo study of diatom assemblages using low temperature method for ESEM

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    SYSNO ASEP0481582
    Document TypeC - Proceedings Paper (int. conf.)
    R&D Document TypeConference Paper
    TitleIn vivo study of diatom assemblages using low temperature method for ESEM
    Author(s) Tihlaříková, Eva (UPT-D) RID, ORCID, SAI
    Neděla, Vilém (UPT-D) RID, ORCID, SAI
    Fránková, Markéta (BU-J) RID, ORCID
    Number of authors3
    Source Title13th Multinational Congress on Microscopy: Book of Abstracts. - Zagreb : Ruder Bošković Institute, Croatian Microscopy Society, 2017 / Gajović A. ; Weber I. ; Kovačević G. ; Čadež V. ; Šegota S. ; Peharec Štefanić P. ; Vidoš A. - ISBN 978-953-7941-19-2
    Pagess. 64-65
    Number of pages2 s.
    Publication formPrint - P
    ActionMultinational Congress on Microscopy /13./
    Event date24.09.2017 - 29.09.2017
    VEvent locationRovinj
    CountryHR - Croatia
    Event typeWRD
    Languageeng - English
    CountryHR - Croatia
    KeywordsESEM ; diatom ; morphology ; LTM
    Subject RIVJA - Electronics ; Optoelectronics, Electrical Engineering
    OECD categoryPlant sciences, botany
    Subject RIV - cooperationInstitute of Botany - Ecology, Behaviour
    R&D ProjectsLO1212 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
    ED0017/01/01 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
    Institutional supportUPT-D - RVO:68081731 ; BU-J - RVO:67985939
    AnnotationDiatoms are the most species-rich algal group represented by 12 000 described species and are recognized as powerful bio indicators and used for water quality monitoring. Diatom taxonomy is predominantly based on the morphology of ornate silicified cell wall called frustule composed of two overlapping parts (thecae). This frustule displays intricate patterns and designs unique to each species. For these studies conventional scanning electron microscopy (SEM) was and still is widely used. This method requires cleaning of diatom frustules in strong acids and peroxides followed by conductive coating.This aggressive procedure removes protoplast and damages delicate structures. Environmental scanning electron microscopy (ESEM) brings advantages of observation of fresh diatom material that are presence of whole intact diatom cells, not only empty diatom frustules, extracellular mucilaginous diatom secrets (e.g. pads, stalks, tubes) and whole diatom assemblages directly in situ together with other algal assemblages (e.g. cyanobacteria or green algae). However, in diatom research, ESEM is used mainly for elimination of conductive coating and observation of these organisms in their native wet state adhered directly on the host plant is not yet common. The ESEM observation of native aquatic samples is usually affected with radiation damage that can be lovered by the use of special methods like the Low Temperature Method (LTM) for the ESEM.
    WorkplaceInstitute of Scientific Instruments
    ContactMartina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178
    Year of Publishing2018
Number of the records: 1  

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