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Metal Tolerance Protein 8 Mediates Manganese Homeostasis and Iron Reallocation during Seed Development and Germination
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SYSNO ASEP 0480505 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Metal Tolerance Protein 8 Mediates Manganese Homeostasis and Iron Reallocation during Seed Development and Germination Author(s) Eroglu, S. (DE)
Giehl, R.F.H. (DE)
Meier, B. (DE)
Takahashi, M. (JP)
Terada, Y. (JP)
Ignatyev, K. (GB)
Andresen, Elisa (BC-A) RID, ORCID
Küpper, Hendrik (BC-A) RID, ORCID
Peiter, E. (DE)
von Wiren, N. (DE)Number of authors 10 Source Title Plant Physiology. - : Oxford University Press - ISSN 0032-0889
Roč. 174, č. 3 (2017), s. 1633-1647Number of pages 15 s. Publication form Print - P Language eng - English Country US - United States Keywords diffusion facilitator family ; arabidopsis-thaliana ; x-ray ; vacuolar manganese Subject RIV CE - Biochemistry OECD category Biochemistry and molecular biology Institutional support BC-A - RVO:60077344 UT WOS 000404233000026 EID SCOPUS 85021801415 DOI 10.1104/pp.16.01646 Annotation Metal accumulation in seeds is a prerequisite for germination and establishment of plants but also for micronutrient delivery to humans. To investigate metal transport processes and their interactions in seeds, we focused on METAL TOLERANCE PROTEIN8 (MTP8), a tonoplast transporter of the manganese (Mn) subclade of cation diffusion facilitators, which in Arabidopsis (Arabidopsis thaliana) is expressed in embryos of seeds. The x-ray fluorescence imaging showed that expression of MTP8 was responsible for Mn localization in subepidermal cells on the abaxial side of the cotyledons and in cortical cells of the hypocotyl. Accordingly, under low Mn availability, MTP8 increased seed stores of Mn, required for efficient seed germination. In mutant embryos lacking expression of VACUOLAR IRON TRANSPORTER1 (VIT1), MTP8 built up iron (Fe) hotspots in MTP8-expressing cells types, suggesting that MTP8 transports Fe in addition to Mn. In mtp8 vit1 double mutant seeds, Mn and Fe were distributed in all cell types of the embryo. An Fe transport function of MTP8 was confirmed by its ability to complement Fe hypersensitivity of a yeast mutant defective in vacuolar Fe transport. Imbibing mtp8-1 mutant seeds in the presence of Mn or subjecting seeds to wet-dry cycles showed that MTP8 conferred Mn tolerance. During germination, MTP8 promoted reallocation of Fe from the vasculature. These results indicate that cell type-specific accumulation of Mn and Fe in seeds depends on MTP8 and that this transporter plays an important role in the generation of seed metal stores as well as for metal homeostasis and germination efficiency under challenging environmental conditions. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2018
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