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Ultralow-Fouling Behavior of Biorecognition Coatings Based on Carboxy-Functional Brushes of Zwitterionic Homo- and Copolymers in Blood Plasma: Functionalization Matters
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SYSNO ASEP 0479511 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Ultralow-Fouling Behavior of Biorecognition Coatings Based on Carboxy-Functional Brushes of Zwitterionic Homo- and Copolymers in Blood Plasma: Functionalization Matters Author(s) Lísalová, Hana (URE-Y) RID
Brynda, Eduard (UMCH-V) RID
Houska, Milan (UMCH-V) RID
Víšová, Ivana (URE-Y)
Mrkvová, Kateřina (URE-Y)
Chadtová Song, Xue (URE-Y)
Gedeonová, Erika (URE-Y)
Surman, František (UMCH-V)
Riedel, Tomáš (UMCH-V) RID, ORCID
Pop-Georgievski, Ognen (UMCH-V) RID, ORCID
Homola, Jiří (URE-Y) RIDNumber of authors 11 Source Title Analytical Chemistry. - : American Chemical Society - ISSN 0003-2700
Roč. 89, č. 6 (2017), s. 3524-3531Number of pages 8 s. Publication form Print - P Language eng - English Country US - United States Keywords Protein adsorption ; Surface platform ; Resistance Subject RIV CD - Macromolecular Chemistry OECD category Polymer science Subject RIV - cooperation Institute of Macromolecular Chemistry - Macromolecular Chemistry R&D Projects GBP205/12/G118 GA ČR - Czech Science Foundation (CSF) LQ1604 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GJ15-09368Y GA ČR - Czech Science Foundation (CSF) Institutional support URE-Y - RVO:67985882 ; UMCH-V - RVO:61389013 UT WOS 000397478300039 EID SCOPUS 85018753537 DOI 10.1021/acs.analchem.6b04731 Annotation Fouling from complex biological fluids such as blood plasma to biorecognition element (BRE)-functionalized coatings hampers the use of affinity biosensor technologies in medical diagnostics. Here, we report the effects the molecular mechanisms involved in functionalization of low-fouling carboxy-functional coatings have on the BRE capacity and resistance to fouling from blood plasma. The specific mechanisms of EDC/NHS activation of carboxy groups, BRE attachment, and deactivation of residual activated groups on recently developed ultra-low-fouling carboxybetaine polymer and copolymer brushes (pCB) as well as conventional carboxy-terminated oligo(ethylene glycol)-based alkanethiolate self-assembled monolayers (OEG-SAMs) are studied using the polarization modulation infrared reflection/absorption spectroscopy, X-ray photoelectron spectroscopy, and surface plasmon resonance methods. It is shown that the fouling resistance of BRE-functionalized pCB coatings is strongly influenced by a deactivation method affecting the ultra-low-fouling molecular structure of the brush and surface charges. It is revealed that, in contrast to free carboxy-group-terminated OEG-SAMs, only a partial deactivation of EDC/NHS-activated zwitterionic carboxy groups by spontaneous hydrolysis is possible in the pCB brushes. The fouling resistance of activated/BREfunctionalized pCB is shown to be recovered only by covalent attachment of amino acid deactivation agents to residual activated carboxy groups of pCB. The developed deactivation procedure is further combined with ultra-low-fouling brushes of random copolymer carboxybetaine methacrylamide (CBMAA) and N-(2-hydroxypropyl) methacrylamide (HPMAA) with optimized CBMAA content (15%) providing a BRE-functionalized coating with superior fouling resistance over various carboxy-functional low-fouling coatings including homopolymer pCB brushes and OEG-SAMs. The biorecognition capabilities of pHPMAACBMAA(15%) are demonstrated via the sensitive label-free detection of a microRNA cancer biomarker (miR-16) in blood plasma Workplace Institute of Radio Engineering and Electronics Contact Petr Vacek, vacek@ufe.cz, Tel.: 266 773 413, 266 773 438, 266 773 488 Year of Publishing 2018
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