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Plant alkaloid sanguinarine and novel potential probiotic strains Lactobacillus apis, Lactobacillus melliventris and Gilliamella apicola promote resistance of honey bees to nematobacterial infection
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SYSNO ASEP 0475943 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Plant alkaloid sanguinarine and novel potential probiotic strains Lactobacillus apis, Lactobacillus melliventris and Gilliamella apicola promote resistance of honey bees to nematobacterial infection Author(s) Hyršl, P. (CZ)
Dobeš, P. (CZ)
Vojtek, L. (CZ)
Hroncová, Z. (CZ)
Tyl, J. (CZ)
Killer, Jiří (UZFG-Y) RID, ORCIDSource Title Bulletin of Insectology. - : Universita degli Studi di Bologna - ISSN 1721-8861
Roč. 70, č. 1 (2017), s. 31-38Number of pages 8 s. Publication form Print - P Language eng - English Country IT - Italy Keywords entomopathogenic nematode ; honey bee ; immunity Subject RIV EG - Zoology OECD category Entomology Institutional support UZFG-Y - RVO:67985904 UT WOS 000399955300005 EID SCOPUS 85018668769 Annotation Entomopathogenic nematodes (EPNs) are obligate insect parasites symbiotically associated with entomopathogenic bacteria. They can be used as a natural infection model combining bacterial infection with infection by multicellular parasite and as such, can be used in biological control and also offer a powerful tool to study insect immunity. Both mix of non-pathogenic bacteria and even nectar alkaloids were previously hypothesized to positively modulate honey bee health. We used EPNs for evaluating the overall immune resistance of honey bee larvae treated with potentially immuno-modulating substances - a plant alkaloid sanguinarine (extracted from Macleaya cordata) and non-pathogenic strains of Lactobacillus apis, Lactobacillus melliventris and Gilliamella apicola, native to honey bee gut isolates. Honey bee 5th instar larvae (L5) and white pupae were infected with Heterorhabditis bacteriophora or Steinernema feltiae, both carrying their symbiotic bacteria. In comparison to untreated honey bee larvae we observed an increase in survival of 13.5 +/- 6.43 or 11.25 +/- 5.77% in case of sanguinarine and S. feltiae or H. bacteriophora, respectively. Similarly, mix of above mentioned bacteria inoculated at the same time increased survivorship to 23.25 +/- 1.53 or 11.0 +/- 6.0% for S. feltiae or H. bacteriophora. This is the first record of use of nematobacterial infection for evaluating the immune status of a beneficial insect. Addition of low doses of both sanguinarine and non-pathogenic strains of selected bacteria had a positive impact on the resistance of honey bee to pathogen. This method can serve as a valuable tool for immunological tests in honey bees. Workplace Institute of Animal Physiology and Genetics Contact Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Year of Publishing 2018
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