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Bridged polysilsesquioxane-based wide-bore monolithic capillary columns for hydrophilic interaction chromatography

  1. 1.
    SYSNO ASEP0469740
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JČlánek ve WOS
    TitleBridged polysilsesquioxane-based wide-bore monolithic capillary columns for hydrophilic interaction chromatography
    Author(s) Šesták, Jozef (UIACH-O) ORCID
    Moravcová, Dana (UIACH-O) RID, ORCID
    Křenková, Jana (UIACH-O) RID, ORCID
    Planeta, Josef (UIACH-O) RID, ORCID
    Roth, Michal (UIACH-O) RID, ORCID
    Foret, František (UIACH-O) RID, ORCID
    Number of authors6
    Source TitleJournal of Chromatography A. - : Elsevier - ISSN 0021-9673
    Roč. 1479, JAN (2017), s. 204-209
    Number of pages6 s.
    Publication formPrint - P
    Languageeng - English
    CountryNL - Netherlands
    Keywordssilica monolithic column ; HILIC ; oligosaccharides ; nucleosides
    Subject RIVCB - Analytical Chemistry, Separation
    OECD categoryAnalytical chemistry
    R&D ProjectsGA14-06319S GA ČR - Czech Science Foundation (CSF)
    GA16-03749S GA ČR - Czech Science Foundation (CSF)
    Institutional supportUIACH-O - RVO:68081715
    UT WOS000392681300023
    EID SCOPUS85008244059
    DOI10.1016/j.chroma.2016.11.062
    AnnotationThe synthesis and characterization of large-bore silica-based monolithic capillary columns (0.32 mm × 150 mm) are presented. Columns were prepared by acidic hydrolysis of a mixture containing tetramethoxysilane (TMOS) and 1,2-bis(trimethoxysilyl)ethane (BTME) in different molar ratios in the presence of polyethylene glycol and urea. The monoliths were modified by zwitterionic monomer [2-(methacryloyloxy)ethyl]-dimethyl-(3-sulfopropyl)-ammonium hydroxide via 3-(trimethoxysilyl)propyl methacrylate. Prepared stationary phases were evaluated by scanning electron microscopy and chromatographic separation of nucleobases and their derivatives in the HILIC mode. The best chromatographic results were obtained with the column prepared from the reaction mixture containing BTME and TMOS in a 1:4 molar ratio. The permeability of such column reached 1.68 × 10−14 m2 and the efficiency, expressed as a height equivalent of the theoretical plate, did not exceed 10.5 μm for the tested compounds. The columns were successfully applied to HILIC separation of native and labeled oligosaccharides and glycans released from bovine ribonuclease B and human immunoglobulin G.
    WorkplaceInstitute of Analytical Chemistry
    ContactIveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234
    Year of Publishing2017
Number of the records: 1  

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