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Bridged polysilsesquioxane-based wide-bore monolithic capillary columns for hydrophilic interaction chromatography
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SYSNO ASEP 0469740 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Bridged polysilsesquioxane-based wide-bore monolithic capillary columns for hydrophilic interaction chromatography Author(s) Šesták, Jozef (UIACH-O) ORCID
Moravcová, Dana (UIACH-O) RID, ORCID
Křenková, Jana (UIACH-O) RID, ORCID
Planeta, Josef (UIACH-O) RID, ORCID
Roth, Michal (UIACH-O) RID, ORCID
Foret, František (UIACH-O) RID, ORCIDNumber of authors 6 Source Title Journal of Chromatography A. - : Elsevier - ISSN 0021-9673
Roč. 1479, JAN (2017), s. 204-209Number of pages 6 s. Publication form Print - P Language eng - English Country NL - Netherlands Keywords silica monolithic column ; HILIC ; oligosaccharides ; nucleosides Subject RIV CB - Analytical Chemistry, Separation OECD category Analytical chemistry R&D Projects GA14-06319S GA ČR - Czech Science Foundation (CSF) GA16-03749S GA ČR - Czech Science Foundation (CSF) Institutional support UIACH-O - RVO:68081715 UT WOS 000392681300023 EID SCOPUS 85008244059 DOI 10.1016/j.chroma.2016.11.062 Annotation The synthesis and characterization of large-bore silica-based monolithic capillary columns (0.32 mm × 150 mm) are presented. Columns were prepared by acidic hydrolysis of a mixture containing tetramethoxysilane (TMOS) and 1,2-bis(trimethoxysilyl)ethane (BTME) in different molar ratios in the presence of polyethylene glycol and urea. The monoliths were modified by zwitterionic monomer [2-(methacryloyloxy)ethyl]-dimethyl-(3-sulfopropyl)-ammonium hydroxide via 3-(trimethoxysilyl)propyl methacrylate. Prepared stationary phases were evaluated by scanning electron microscopy and chromatographic separation of nucleobases and their derivatives in the HILIC mode. The best chromatographic results were obtained with the column prepared from the reaction mixture containing BTME and TMOS in a 1:4 molar ratio. The permeability of such column reached 1.68 × 10−14 m2 and the efficiency, expressed as a height equivalent of the theoretical plate, did not exceed 10.5 μm for the tested compounds. The columns were successfully applied to HILIC separation of native and labeled oligosaccharides and glycans released from bovine ribonuclease B and human immunoglobulin G. Workplace Institute of Analytical Chemistry Contact Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Year of Publishing 2017
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