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Kapilární separace, mikrofluidika a hmotnostní spektrometrie pro bioanalýzu
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SYSNO ASEP 0467152 Document Type A - Abstract R&D Document Type The record was not marked in the RIV R&D Document Type Není vybrán druh dokumentu Title Kapilární separace, mikrofluidika a hmotnostní spektrometrie pro bioanalýzu Author(s) Foret, František (UIACH-O) RID, ORCID
Křenková, Jana (UIACH-O) RID, ORCID
Klepárník, Karel (UIACH-O) RID, ORCID
Týčová, Anna (UIACH-O) ORCID
Partyka, Jan (UIACH-O)Number of authors 5 Source Title Sborník přednášek a posterů XXV. Biochemický sjezd. - Praha : Česká společnost pro biochemii a molekulární biologii, 2016 / Pačes Václav ; Stiborová Marie ; Turňa Ján Number of pages 1 s. Publication form Print - P Action Biochemický sjezd /25./ Event date 13.09.2016 - 16.09.2016 VEvent location Praha Country CZ - Czech Republic Event type EUR Language eng - English Country CZ - Czech Republic Keywords microfabricated liquid junction ; electrospray tip ; electrospray ionization Subject RIV CB - Analytical Chemistry, Separation Institutional support UIACH-O - RVO:68081715 Annotation In this work we have explored two, application dependent, approaches. A hybrid design, utilizing a microfabricated liquid junction/sprayer module, allowed coupling to a commercial, fully automated, CE analyser equipped with standard (50-75 m ID) separation capillaries. In the second, “interface-free” approach, the CE/MS analysis was performed in a narrow bore (<20 m ID) separation capillary forming one entity with the electrospray tip. The high voltage, applied at the injection end of the capillary, served for both the separation and electrospray ionization. Optimum conditions for the separation and electrospray ionization were achieved with voltage programming. The performance of both CE/MS systems was tested for separations of metabolites, peptides and glycoproteins and compared to other designs with respect to the separation efficiency, sensitivity and sample consumption. Workplace Institute of Analytical Chemistry Contact Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Year of Publishing 2017
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