Aspergillus infection monitored by multimodal imaging in a rat model

0462011 - MBÚ 2017 RIV US eng J - Journal Article
Pluháček, Tomáš - Petrík, M. - Luptáková, Dominika - Benada, Oldřich - Palyzová, Andrea - Lemr, Karel - Havlíček, Vladimír
Aspergillus infection monitored by multimodal imaging in a rat model.
Proteomics. Roč. 16, 11-12 (2016), s. 1785-1792. ISSN 1615-9853. E-ISSN 1615-9861
R&D Projects: GA MŠMT LO1509; GA ČR GAP206/12/1150
Institutional support: RVO:61388971
Keywords : Animal model * Aspergillosis * Biomedicine
Subject RIV: CE - Biochemistry
Impact factor: 4.041, year: 2016

Although myriads of experimental approaches have been published in the field of fungal infection diagnostics, interestingly, in 21st century there is no satisfactory early noninvasive tool for Aspergillus diagnostics with good sensitivity and specificity. In this work, we for the first time described the fungal burden in rat lungs by multimodal imaging approach. The Aspergillus infection was monitored by positron emission tomography and light microscopy employing modified Grocott's methenamine silver staining and eosin counterstaining. Laser ablation inductively coupled plasma mass spectrometry imaging has revealed a dramatic iron increase in fungi-affected areas, which can be presumably attributed to microbial siderophores. Quantitative elemental data were inferred from matrix-matched standards prepared from rat lungs.
Permanent Link: http://hdl.handle.net/11104/0261546