Number of the records: 1
Effect of metal ions on autofluorescence of the dry rot fungus Serpula lacrymans grown on spruce wood
- 1.
SYSNO ASEP 0460038 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Effect of metal ions on autofluorescence of the dry rot fungus Serpula lacrymans grown on spruce wood Author(s) Gabriel, Jiří (MBU-M) RID
Žižka, Zdeněk (MBU-M) RID, ORCID
Švec, Karel (MBU-M) ORCID
Nasswettrová, A. (CZ)
Šmíra, P. (CZ)
Kofroňová, Olga (MBU-M) RID, ORCID
Benada, Oldřich (MBU-M) ORCID, RIDSource Title Folia Microbiologica. - : Springer - ISSN 0015-5632
Roč. 61, č. 2 (2016), s. 119-128Number of pages 10 s. Language eng - English Country CZ - Czech Republic Keywords HEAVY-METALS ; CELL-WALL ; PHANEROCHAETE-CHRYSOSPORIUM Subject RIV EE - Microbiology, Virology Institutional support MBU-M - RVO:61388971 UT WOS 000370185200004 EID SCOPUS 84958102161 DOI 10.1007/s12223-015-0415-x Annotation This work describes autofluorescence of the mycelium of the dry rot fungus Serpula lacrymans grown on spruce wood blocks impregnated with various metals. Live mycelium, as opposed to dead mycelium, exhibited yellow autofluorescence upon blue excitation, blue fluorescence with ultraviolet (UV) excitation, orange-red and light-blue fluorescence with violet excitation, and red fluorescence with green excitation. Distinctive autofluorescence was observed in the fungal cell wall and in granula localized in the cytoplasm. In dead mycelium, the intensity of autofluorescence decreased and the signal was diffused throughout the cytoplasm. Metal treatment affected both the color and intensity of autofluorescence and also the morphology of the mycelium. The strongest yellow signal was observed with blue excitation in Cd-treated samples, in conjunction with increased branching and the formation of mycelial loops and protrusions. For the first time, we describe pink autofluorescence that was observed in Mn-, Zn-, and Cu-treated samples with UV, violet or. blue excitation. The lowest signals were obtained in Cu- and Fe-treated samples. Chitin, an important part of the fungal cell wall exhibited intensive primary fluorescence with UV, violet, blue, and green excitation. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2017
Number of the records: 1