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Fast evolutionary rates associated with functional loss in class I glucose transporters of Schistosoma mansoni
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SYSNO ASEP 0453234 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Fast evolutionary rates associated with functional loss in class I glucose transporters of Schistosoma mansoni Author(s) Cabezas-Cruz, A. (FR)
Valdés, James J. (BC-A) RID, ORCID
Lancelot, J. (FR)
Pierce, R.J. (FR)Source Title BMC Genomics. - : BioMed Central - ISSN 1471-2164
Roč. 16, NOV 19 2015 (2015), s. 980Number of pages 18 s. Publication form Online - E Language eng - English Country US - United States Keywords Schistosoma mansoni ; glucose transporters ; transcriptional regulation ; phylogen ; biophysics Subject RIV EI - Biotechnology ; Bionics R&D Projects EE2.3.30.0032 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support BC-A - RVO:60077344 UT WOS 000365284800003 DOI 10.1186/s12864-015-2144-6 Annotation By modeling the phase of human host infection, we showed that transporter transcript expression profiles of recently transformed schistosomula have two opposing responses to increased glucose concentrations. Concurring with the transcription profiles, our phylogenetic analyses revealed that S. mansoni glucose transporters belong to two separate clusters, one associated with class I glucose transporters from vertebrates and insects, and the other specific to parasitic Platyhelminthes. To study the evolutionary paths of both groups and their functional implications, we determined evolutionary rates, relative divergence times, genomic organization and performed structural analyses with the protein sequences. We finally used the modelled structures of the S. mansoni glucose transporters to biophysically (i) analyze the dynamics of key residues during glucose binding, (ii) test glucose stability within the active site, and (iii) demonstrate glucose diffusion. The two S. mansoni Platyhelminthes-specific glucose transporters, which seem to be younger than the other two, exhibit slower rates of molecular evolution, are encoded by intron-poor genes, and transport glucose. Interestingly, our molecular dynamic analyses suggest that S. mansoni class I glucose transporters are not able to transport glucose. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2016
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