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Scanning electron microscopy and Raman spectroscopy of microorganisms related to biofuels and biopolymer production
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SYSNO ASEP 0452285 Document Type C - Proceedings Paper (int. conf.) R&D Document Type The record was not marked in the RIV Title Scanning electron microscopy and Raman spectroscopy of microorganisms related to biofuels and biopolymer production Author(s) Hrubanová, Kamila (UPT-D) RID, SAI, ORCID
Samek, Ota (UPT-D) RID, ORCID, SAI
Obruča, S. (CZ)
Hároniková, A. (CZ)
Krzyžánek, Vladislav (UPT-D) RID, ORCID, SAINumber of authors 5 Source Title MC 2015. Microscopy Conference Proceedings. - Göttingen : DGE, 2015 Pages s. 700-701 Number of pages 2 s. Publication form Online - E Action Microscopy Conference 2015 Event date 06.09.2015-11.09.2015 VEvent location Göttingen Country DE - Germany Event type WRD Language eng - English Country DE - Germany Keywords SEM ; Raman spectroscopy Subject RIV JA - Electronics ; Optoelectronics, Electrical Engineering R&D Projects GA14-20012S GA ČR - Czech Science Foundation (CSF) LO1212 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) ED0017/01/01 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UPT-D - RVO:68081731 Annotation Red yeast strains such as Rhodotorula spp., Cystofilobasidium spp. and Sporobolomyces spp. are well known due to their potential applications in the field of biofuel generation and food industry. In order to utilize biomass for efficient industrial production, the optimal cultivation parameters have to be determined which in turn lead to high production of desired substances such as oil and carotenoids in the selected cell line. In this project we have studied, using scanning electron microscopy (SEM) and Raman spectroscopy techniques, how different cultivation conditions influence production of oil and carotenoids. Raman spectroscopy can be used for the determination of the oil present in the biomass and also for the determination of carotenoids as the intensity ratios of specific, selected Raman bands. SEM uses focused electron beam to gain information about morphology of cells (biomass structure) which is very important factor to study cells response on the applied stress. Workplace Institute of Scientific Instruments Contact Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178 Year of Publishing 2016
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