Methodological Aspects of In Vitro Assessment of Bio-accessible Risk Element Pool in Urban Particulate Matter

0434532 - FGÚ 2015 RIV US eng J - Journal Article
Sysalová, J. - Száková, J. - Tremlová, J. - Kašparovská, Kateřina - Kotlík, B. - Tlustoš, P. - Svoboda, Petr
Methodological Aspects of In Vitro Assessment of Bio-accessible Risk Element Pool in Urban Particulate Matter.
Biological Trace Element Research. Roč. 161, č. 2 (2014), s. 216-222. ISSN 0163-4984. E-ISSN 1559-0720
Grant - others:GA ČR(CZ) GA521/09/1150; GA ČR(CZ) GAP503/12/0682
Program: GA; GA
Institutional support: RVO:67985823
Keywords : risk elements * urban particulate matter * in vitro tests * bio-accessibility
Subject RIV: CB - Analytical Chemistry, Separation
Impact factor: 1.748, year: 2014

In vitro tests simulating the elements release from inhaled urban particulate matter with artificial lung fluids and simulated gastric and pancreatic solutions were applied for an estimation of hazardous element bioaccessibility in this material. An inductively coupled plasma optical emission spectrometry (ICP-OES) and an inductively coupled plasma mass spectrometry (ICP-MS) were employed for the element determination in extracted solutions. The effect of the extraction agent used, extraction time, sample-to-extractant ratio, sample particle size and/or individual element properties was evaluated. Results of simulated in vitro tests can be applied for the reasonable estimation of bio-accessible element portions in the particulate matter as an alternative method, which, consequently, initiates further examinations including potential in vivo assessments
Permanent Link: http://hdl.handle.net/11104/0238555