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Changes in the expression of selected testicular genes in mice
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SYSNO ASEP 0428740 Document Type K - Proceedings Paper (Czech conf.) R&D Document Type The record was not marked in the RIV Title Changes in the expression of selected testicular genes in mice Author(s) Valášková, Eliška (BTO-N)
Margaryan, Hasmik (BTO-N) RID
Žatecká, Eva (BTO-N)
Pěknicová, Jana (BTO-N) RIDNumber of authors 4 Source Title Book of abstracts XXth Symposium of Biology and Immunology of Reproduction with international participation. - Praha : Biotechnologický ústav AVČR, v.v.i, 2014 / Pěknicová Jana
S. 1-82Number of pages 1 s. Action XXth Symposium of Biology and Immunology of Reproduction with international participation Event date 22.05.2014-24.05.2014 VEvent location Třešť Country CZ - Czech Republic Event type WRD Language eng - English Country CZ - Czech Republic Keywords diabetes mellitus ; gene expression ; reproduction Subject RIV EB - Genetics ; Molecular Biology R&D Projects P503/12/1834 GA ČR - Czech Science Foundation (CSF) CZ1.05/1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support BTO-N - RVO:86652036 CEZ AV0Z50520701 - BTO-N (2007-2013) Annotation The decrease in population fertility has become a major concern in many developed countries. Recent studies show that infertility is affecting an estimated 15% of all couples (World Health Organization, WHO, 2010). Male infertility is the primary or contributing cause in 60% of cases. Male infertility is caused by a number of factors, such as genetic background, various environmental factors and disease. Diabetes mellitus (DM), a serious health problem on its own, is also suspected to be a contributing factor to male infertility. The aim of this project was to analyze the cellular, molecular and genetic effects of diabetic environment on spermatogenesis and sperm quality and to determine the impact of DM on the in vivo reproduction, using the mouse model (Mus musculus) inbred FVB. Diabetes was induced using streptozotocin. We used our knowledge and tools (unique monoclonal antibodies developed by our group) to determine the status of reproductive organs, anogenital distance, and the quality of sperms. Genetic analysis was performed by a quantitative Reverse Transcription Polymerase Chain Reaction (qPCR). We tested selected genes which are expressed in testicular tissue and thus can influence process of spermatogenesis and consequently the sperm quality. Our preliminary data strongly suggest that DM impairs male fertility. We have found significant changes in the body and reproductive organ weight of mice with DM. We have identified qualitative and quantitative changes in the expression of proteins in epididymal fluid and sperms. We have also detected an increased number of apoptotic cells in sperm of diabetic mice compared to the control group. To our knowledge, there is no study assessing the correlation between DM and “unexplained infertility”. In view of this, it is essential to analyze the effects of DM on male fertility, sperm quality, and reproduction parameters. Workplace Institute of Biotechnology Contact Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Year of Publishing 2015
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