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SIKVAV-modified highly superporous PHEMA scaffolds with oriented pores for spinal cord injury repair
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SYSNO ASEP 0397919 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title SIKVAV-modified highly superporous PHEMA scaffolds with oriented pores for spinal cord injury repair Author(s) Kubinová, Šárka (UEM-P) RID, ORCID
Horák, Daniel (UMCH-V) RID, ORCID
Hejčl, Aleš (UEM-P) RID, ORCID
Plichta, Zdeněk (UMCH-V)
Kotek, Jiří (UMCH-V) RID
Proks, Vladimír (UMCH-V) RID, ORCID
Forostyak, Serhiy (UEM-P) RID, ORCID
Syková, Eva (UEM-P) RIDNumber of authors 8 Source Title Journal of Tissue Engineering and Regenerative Medicine. - : Wiley - ISSN 1932-6254
Roč. 9, č. 11 (2015), s. 1298-1309Number of pages 12 s. Language eng - English Country GB - United Kingdom Keywords hydrogel ; oriented pores ; spinal cord repair Subject RIV FH - Neurology Subject RIV - cooperation Institute of Macromolecular Chemistry - Neurology R&D Projects GAP304/11/0731 GA ČR - Czech Science Foundation (CSF) GPP304/11/P633 GA ČR - Czech Science Foundation (CSF) GAP108/10/1560 GA ČR - Czech Science Foundation (CSF) GAP304/11/0653 GA ČR - Czech Science Foundation (CSF) Institutional support UEM-P - RVO:68378041 ; UMCH-V - RVO:61389013 UT WOS 000364520300010 EID SCOPUS 84946495826 DOI 10.1002/term.1694 Annotation The architecture and mechanical properties of a scaffold for spinal cord injury treatment must provide tissue integration as well as effective axonal regeneration. Previous work has demonstrated the cell-adhesive and growth-promoting properties of the SIKVAV (Ser-Ile-Lys-Val-Ala-Val)-modified highly superporous poly(2-hydroxethyl methacrylate) (PHEMA) hydrogels. The aim of the current study was to optimize the porosity and mechanical properties of this type of hydrogel in order to develop a suitable scaffold for the repair of spinal cord tissue. Three types of highly superporous PHEMA hydrogels with oriented pores of similar to 60 mu m diameter, porosities of 57-68% and equivalent stiffness characterized by elasticity moduli in the range 3-45 kPa were implanted into a spinal cord hemisection, and their integration into the host tissue, as well as the extent of axonal ingrowth into the scaffold pores, were histologically evaluated. The best tissue response was found with a SIKVAV-modified PHEMA hydrogel with 68% porosity and a moderate modulus of elasticity (27 kPa in the direction along the pores and 3.6 kPa in the perpendicular direction). When implanted into a spinal cord transection, the hydrogel promoted tissue bridging as well as aligned axonal ingrowth. In conclusion, a prospective oriented scaffold architecture of SIKVAV-modified PHEMA hydrogels has been developed for spinal cord injury repair; however, to develop an effective treatment for spinal cord injury, multiple therapeutic approaches are needed. Workplace Institute of Experimental Medicine Contact Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Year of Publishing 2016
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