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Toxicity of complex cyanobacterial samples and their fractions in Xenopus laevis embryos and the role of microcystins

  1. 1.
    SYSNO ASEP0358743
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JČlánek ve WOS
    TitleToxicity of complex cyanobacterial samples and their fractions in Xenopus laevis embryos and the role of microcystins
    Author(s) Buryšková, B. (CZ)
    Hilscherová, Klára (BU-J)
    Babica, Pavel (BU-J) RID, ORCID
    Vršková, D. (CZ)
    Maršálek, Blahoslav (BU-J) ORCID, SAI
    Bláha, Luděk (BU-J)
    Number of authors6
    Source TitleAquatic Toxicology. - : Elsevier - ISSN 0166-445X
    Roč. 80, č. 4 (2006), s. 346-354
    Number of pages9 s.
    Languageeng - English
    CountryNL - Netherlands
    KeywordsFETAX ; Xenopus laevis ; malformations ; cyanobacterial fractions ; biomarkers
    Subject RIVEF - Botanics
    R&D Projects1M0571 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
    KJB6005411 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
    CEZAV0Z60050516 - BU-J (2005-2011)
    UT WOS000243779900004
    DOI10.1016/j.aquatox.2006.10.001
    AnnotationThe object of this work was to evaluate the effects of various cyanobacterial fractions in Frog Embryo Teratogenesis Assay Xenopus (FETAX) with African clawed frog embryos. Fractions were prepared from five biomasses with different dominant genera (Microcystis, Aphanizomenon, Anabaena, Planktothrix) and different microcystin content. Effects of following fractions were investigated: (I) homogenate of complex cyanobacterial biomass, (II) cell debris (pellet) after centrifugation of complex biomass, (III) supernatant after centrifugation of complex biomass (= crude aqueous extract), (IV) permeate after passing of crude extract through C-18 column (fraction devoid of microcystins), (V) eluate from C-18 column (containing microcysins, if present).
    WorkplaceInstitute of Botany
    ContactMartina Bartošová, martina.bartosova@ibot.cas.cz, ibot@ibot.cas.cz, Tel.: 271 015 242 ; Marie Jakšová, marie.jaksova@ibot.cas.cz, Tel.: 384 721 156-8
    Year of Publishing2012
Number of the records: 1  

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