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Shielding effect of monovalent and divalent cations on solid-phase DNA hybridization: surface plasmon resonance biosensor study
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SYSNO ASEP 0350303 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Shielding effect of monovalent and divalent cations on solid-phase DNA hybridization: surface plasmon resonance biosensor study Author(s) Špringer, Tomáš (URE-Y)
Šípová, Hana (URE-Y)
Vaisocherová, Hana (URE-Y) RID
Štepánek, J. (CZ)
Homola, Jiří (URE-Y) RIDNumber of authors 5 Source Title Nucleic Acids Research. - : Oxford University Press - ISSN 0305-1048
Roč. 38, č. 20 (2010), s. 7343-7351Number of pages 9 s. Language eng - English Country GB - United Kingdom Keywords oligonucleotide ; monovalent and divalent cations ; surface plasmon resonance Subject RIV BO - Biophysics R&D Projects KAN200670701 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) CEZ AV0Z20670512 - URE-Y (2005-2011) UT WOS 000284165100051 DOI 10.1093/nar/gkq577 Annotation We investigated the simultaneous effect of monovalent and divalent cations on hybridization of DNA targets to DNA probes immobilized on the surface of a surface plasmon resonance sensor. Our results demonstrated that divalent magnesium is found to be much more efficient in duplex stabilization than monovalent sodium. This trend is opposite to that established for oligonucleotides in a solution. Moreover, the selected saline composition of buffer improved the discrimination of complementary and point mismatched DNA targets. Workplace Institute of Radio Engineering and Electronics Contact Petr Vacek, vacek@ufe.cz, Tel.: 266 773 413, 266 773 438, 266 773 488 Year of Publishing 2011
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