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Ultrastructural and nuclear antigen preservation after high-pressure freezing/freeze-substitution and low-temperature LR White embedding of HeLa cells
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SYSNO ASEP 0315843 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Ultrastructural and nuclear antigen preservation after high-pressure freezing/freeze-substitution and low-temperature LR White embedding of HeLa cells Title Ultrastruktura a detekce jaderných antigenů v HeLa buňkách zalitých do LR White po vysokotlakém zamražení a mrazové substituci Author(s) Strádalová, Vendula (UEM-P) RID
Kyselá, Katarína (UEM-P)
Hozák, Pavel (UMG-J) RID, ORCIDSource Title Histochemistry and Cell Biology. - : Springer - ISSN 0948-6143
Roč. 130, - (2008), s. 1047-1052Number of pages 6 s. Language eng - English Country DE - Germany Keywords Cryofixation ; Freeze substitution ; High-presszre freezing Subject RIV EA - Cell Biology R&D Projects KAN200520704 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) LC545 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) 2B06063 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GD204/05/H023 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50390512 - UEM-P (2005-2011) AV0Z50520514 - UMG-J (2005-2011) UT WOS 000259997900020 DOI 10.1007/s00418-008-0504-x Annotation This article presents new protocol for high pressure freezing and freeze substitution of mammalian cells grown in suspension suitable for immuno-electron microscopy. It shows the suitability of LR White resin usage for ultrastructure and antigen preservation of cells that were not chemically fixed. Workplace Institute of Experimental Medicine Contact Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Year of Publishing 2009
Number of the records: 1