Molecular cloning of the prothoracicotropic hormone from the tobacco hornworm, Manduca sexta
1.
SYSNO ASEP
0157661
Document Type
J - Journal Article
R&D Document Type
Journal Article
Subsidiary J
Ostatní články
Title
Molecular cloning of the prothoracicotropic hormone from the tobacco hornworm, Manduca sexta
Author(s)
Shionoya, M. (JP) Matsubayashi, H. (JP) Asahina, Masako (PAU-O) Kuniyoshi, H. (JP) Nagata, S. (JP) Riddiford, L. M. (US) Kataoka, H. (JP)
Source Title
Insect Biochemistry and Molecular Biology. - : Elsevier
- ISSN 0965-1748
Roč. 33, č. 8 (2003), s. 795-801
Number of pages
7 s.
Language
eng - English
Country
GB - United Kingdom
Keywords
prothoracicotropic hormone ; PTTH ; Manduca sexta
Subject RIV
EB - Genetics ; Molecular Biology
CEZ
AV0Z6022909 - PAU-O, BC-A
Annotation
A cDNA encoding a putative precursor of prothoracicotropic hormone (PTTH) from the tobacco hornworm, Manduca sexta, was isolated and sequenced. This clone contains an open reading frame encoding a 226-amino acid prepropeptide hormone. The deduced amino acid sequence is composed of a signal sequence, a precursor domain and a mature hormone and shows similarities to the other PTTHs that have been cloned from closely related lepidopteran species, Bombyx mori, Samia cynthia ricini, Antheraea peryni, and Hyalophora cecropia. The recombinant putative Manduca PTTH produced in E. coli was biologically active, both causing a larval molt in neck-ligated Manduca 4th instar larvae (ED50=50 pM) and the adult molt of diapausing Manduca pupae (ED50=79 pM), but was unable to stimulate molting of debrained Bombyx pupae.
