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Effects of interferon gamma and specific polyclonal antibody on the infection of murine peritoneal macrophages and murine macrophage cell line PMJ2-R with Encephalitozoon cuniculi
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SYSNO ASEP 0089125 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Effects of interferon gamma and specific polyclonal antibody on the infection of murine peritoneal macrophages and murine macrophage cell line PMJ2-R with Encephalitozoon cuniculi Title Vliv interferonu gama a specifických polyklonálních protilátek na vývoj infekce Encephalitozoon cuniculi v myších peritoneálních makrofázích a v buněčné linii PMJ2-R Author(s) Jelínek, Jiří (BC-A)
Salát, Jiří (BC-A) RID, ORCID
Sak, Bohumil (BC-A) RID, ORCID
Kopecký, Jan (BC-A) RIDSource Title Folia Parasitologica. - : Biologické centrum AV ČR, v. v. i. - ISSN 0015-5683
Roč. 54, č. 3 (2007), s. 172-176Number of pages 5 s. Language eng - English Country CZ - Czech Republic Keywords microsporidia ; Encephalitozoon cuniculi ; antibody ; macrophages ; interferon gamma (IFN-gamma) Subject RIV EC - Immunology R&D Projects GP524/03/D167 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z60220518 - PAU-O, BC-A (2005-2011) Annotation Activation of peritoneal macrophages by interferon gamma (IFN-gamma) resulted in the inhibition of Encephalitozoon cuniculi replication. E. cuniculi could replicate either in a non-activated cell line of murine macrophages PMJ2-R or in IFN-gamma-activated PMJ2-R cells. Moreover, activation with IFN-gamma led to faster replication of E. cuniculi in these cells. Opsonisation of E. cuniculi spores with anti-E. cuniculi polyclonal antibody did not affect E. cuniculi replication in both, non-activated and activated murine macrophages. Opsonisation of E. cuniculi spores caused the most effective replication of E. cuniculi in activated PMJ2-R cells. Results support the hypothesis that E. cuniculi uses phagocytosis for the infection of host cells. They also indicate that the mechanism by which spores of E. cuniculi are killed by macrophages is not dependent on nitric oxide and they reveal that PMJ2-R cells cannot substitute peritoneal murine macrophages in immunological studies on E. cuniculi. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2008
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