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Plant Synthetic Promoters: Advancement and Prospective
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SYSNO ASEP 0584252 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Plant Synthetic Promoters: Advancement and Prospective Author(s) Khan, Ahamed (BC-A) RID
Nasim, N. (IN)
Pudhuvai, B. (CZ)
Koul, B. (IN)
Upadhyay, S. K. (IN)
Sethi, L. (IN)
Dey, N. (IN)Number of authors 7 Article number 298 Source Title Agriculture-Basel. - : MDPI
Roč. 13, č. 2 (2023)Number of pages 21 s. Language eng - English Country CH - Switzerland Keywords synthetic promoter ; crispr ; Cis-engineering ; transcription factors ; gene expression Subject RIV EB - Genetics ; Molecular Biology OECD category Genetics and heredity (medical genetics to be 3) Method of publishing Open access Institutional support BC-A - RVO:60077344 UT WOS 000938627500001 EID SCOPUS 85149121902 DOI 10.3390/agriculture13020298 Annotation Native/endogenous promoters have several fundamental limitations in terms of their size, Cis-elements distribution/patterning, and mode of induction, which is ultimately reflected in their insufficient transcriptional activity. Several customized synthetic promoters were designed and tested in plants during the past decade to circumvent such constraints. Such synthetic promoters have a built-in capacity to drive the expression of the foreign genes at their maximum amplitude in plant orthologous systems. The basic structure and function of the promoter have been discussed in this review, with emphasis on the role of the Cis-element in regulating gene expression. In addition to this, the necessity of synthetic promoters in the arena of plant biology has been highlighted. This review also provides explicit information on the two major approaches for developing plant-based synthetic promoters: the conventional approach (by utilizing the basic knowledge of promoter structure and Cis-trans interaction) and the advancement in gene editing technology. The success of plant genetic manipulation relies on the promoter efficiency and the expression level of the transgene. Therefore, advancements in the field of synthetic promoters has enormous potential in genetic engineering-mediated crop improvement. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2024 Electronic address https://www.mdpi.com/2077-0472/13/2/298
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