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SERS-Tags: Selective Immobilization and Detection of Bacteria by Strain-Specific Antibodies and Surface-Enhanced Raman Scattering
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SYSNO ASEP 0570271 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title SERS-Tags: Selective Immobilization and Detection of Bacteria by Strain-Specific Antibodies and Surface-Enhanced Raman Scattering Author(s) Benešová, Markéta (UPT-D)
Bernatová, Silvie (UPT-D) RID, SAI
Mika, Filip (UPT-D) RID, SAI, ORCID
Pokorná, Zuzana (UPT-D) RID, ORCID, SAI
Ježek, Jan (UPT-D) RID, ORCID, SAI
Šiler, Martin (UPT-D) RID, ORCID, SAI
Samek, Ota (UPT-D) RID, ORCID, SAI
Růžička, F. (CZ)
Rebrošová, K. (CZ)
Zemánek, Pavel (UPT-D) RID, SAI, ORCID
Pilát, Zdeněk (UPT-D) RID, SAI, ORCIDNumber of authors 11 Article number 182 Source Title Biosensors. - : MDPI
Roč. 13, č. 2 (2023)Number of pages 14 s. Publication form Online - E Language eng - English Country CH - Switzerland Keywords SERS-tag ; Escherichia coli ; sandwich immunoassay ; single-cell detection Subject RIV EE - Microbiology, Virology OECD category Microbiology R&D Projects NU21-05-00341 GA MZd - Ministry of Health (MZ) Research Infrastructure Czech-BioImaging III - 90250 - Ústav molekulární genetiky AV ČR, v. v. i. Method of publishing Open access Institutional support UPT-D - RVO:68081731 UT WOS 000938961100001 EID SCOPUS 85149147304 DOI 10.3390/bios13020182 Annotation Efficient separation and sensitive identification of pathogenic bacterial strains is essential for a prosperous modern society, with direct applications in medical diagnostics, drug discovery, biodefense, and food safety. We developed a fast and reliable method for antibody-based selective immobilization of bacteria from suspension onto a gold-plated glass surface, followed by detection using strain-specific antibodies linked to gold nanoparticles decorated with a reporter molecule. The reporter molecules are subsequently detected by surface-enhanced Raman spectroscopy (SERS). Such a multi-functionalized nanoparticle is called a SERS-tag. The presented procedure uses widely accessible and cheap materials for manufacturing and functionalization of the nanoparticles and the immobilization surfaces. Here, we exemplify the use of the produced SERS-tags for sensitive single-cell detection of opportunistic pathogen Escherichia coli, and we demonstrate the selectivity of our method using two other bacterial strains, Staphylococcus aureus and Serratia marcescens, as negative controls. We believe that the described approach has a potential to inspire the development of novel medical diagnostic tools for rapid identification of bacterial pathogens. Workplace Institute of Scientific Instruments Contact Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178 Year of Publishing 2024 Electronic address https://www.mdpi.com/2079-6374/13/2/182
Number of the records: 1