Mixed Infection of Blackcurrant with a Novel Cytorhabdovirus and Black Currant-Associated Nucleorhabdovirus

Petrzik, Karel; Přibylová, Jaroslava; Špak, Josef; Sarkisova, Tatiana; Fránová, Jana; Holub, J.; Skalík, J.; Koloniuk, Igor

doi:https://dx.doi.org/10.3390/v14112456

Number of the records: 1

Mixed Infection of Blackcurrant with a Novel Cytorhabdovirus and Black Currant-Associated Nucleorhabdovirus

1.

SYSNO ASEP	0568777
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Mixed Infection of Blackcurrant with a Novel Cytorhabdovirus and Black Currant-Associated Nucleorhabdovirus
Author(s)	Petrzik, Karel (BC-A)_{RID, ORCID} Přibylová, Jaroslava (BC-A)_{RID, ORCID} Špak, Josef (BC-A)_{RID, ORCID} Sarkisova, Tatiana (BC-A)_RID Fránová, Jana (BC-A)_{RID, ORCID} Holub, J. (CZ) Skalík, J. (CZ) Koloniuk, Igor (BC-A)_{RID, ORCID}
Number of authors	8
Article number	2456
Source Title	Viruses. - : MDPI Roč. 14, č. 11 (2022)
Number of pages	10 s.
Language	eng - English
Country	CH - Switzerland
Keywords	aphid transmission ; blackcurrant rhabdovirus 2 ; blackcurrant-associated rhabdovirus ; electron microscopy ; high throughput sequencing ; Ribes nigrum
Subject RIV	EE - Microbiology, Virology
OECD category	Virology
R&D Projects	LM2015062 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	EF16_013/0001775 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Method of publishing	Open access
Institutional support	BC-A - RVO:60077344
UT WOS	000910817000001
DOI	10.3390/v14112456
Annotation	A virome screen was performed on a new breeding line, KB1, of blackcurrant. Rhabdovirus-like particles were observed by electron microscopy in ultrathin sections of flower stalks, and the complete genome sequence of a novel virus, provisionally named blackcurrant rhabdovirus 2 (BCRV2), was determined and verified using high-throughput sequencing. The genomic organization of BCRV2 was characteristic of cytorhabdoviruses (family Rhabdoviridae) and included seven genes: 3 '-N-P '-P-P3-M-G-L-5 '. BLASTP analysis revealed that the putative L protein had the highest amino acid sequence identity (75%) with strawberry virus 2. BCRV2 was detected in Cryptomyzus galeopsidis, but efficient transmission by this aphid was not confirmed. Of note, we observed coinfection of the KB1 line with blackcurrant-associated rhabdovirus (BCaRV) by RT-PCR. This is likely the first evidence of the presence of a cyto- and a nucleorhabdovirus in a single host.
Workplace	Biology Centre (since 2006)
Contact	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Year of Publishing	2023
Electronic address	https://www.mdpi.com/1999-4915/14/11/2456

Number of the records: 1