Acinetobacter amyesii sp. nov., widespread in the soil and water environment and animals

0564932 - MBÚ 2023 RIV GB eng J - Journal Article
Němec, A. - Radolfová-Křížová, L. - Maixnerová, M. - Němec, M. - Shestivska, V. - Španělová, P. - Kyselková, Martina - Wilharm, G. - Higgins, P. G.
Acinetobacter amyesii sp. nov., widespread in the soil and water environment and animals.
International Journal of Systematic and Evolutionary Microbiology. Roč. 72, č. 10 (2022), č. článku 005642. ISSN 1466-5026. E-ISSN 1466-5034
R&D Projects: GA ČR(CZ) GA22-05373S
Research Infrastructure: e-INFRA CZ - 90140
Institutional support: RVO:61388971
Keywords : carbon source assimilation * core genome phylogeny * maldi-tof ms * whole genome sequence
OECD category: Microbiology
Impact factor: 2.8, year: 2022
Method of publishing: Open access
https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.005642

We studied a novel taxon of the genus Acinetobacter , which comprised six strains collected in Czechia, Germany, Indonesia and Turkey between 2015 and 2021. The organisms were isolated from environmental soil, water samples and cow faeces. Their genome sizes varied between 3.3 and 3.5 Mb, with a G+C content of 40.4–40.8 mol%. Based on genus-wide core genome analysis, the taxon formed a distinct clade, with Acinetobacter gandensis being the phylogenetically closest related species. The intrataxon genomic average nucleotide identity based on blast (ANIb) and digital DNA–DNA hybridization (dDDH) values reached 95.3–97.4% and 62.5–77.8 %, respectively, whereas its ANIb/dDDH values against the known Acinetobacter type strains were ≤82.7 %/≤25.7 %. Cluster analysis of whole-cell MALDI-TOF mass spectra corroborated the distinctness and cohesiveness of the taxon. The novel strains were non-glucose-oxidizing, non-haemolytic and non-proteolytic, growing at up to 37–41 °C but not at 44 °C and utilizing 8–10 of the 36 carbon sources tested. Growth on glutarate, tricarballylate and at 37 °C combined with the inability to assimilate 4-aminobutyrate and d-malate differentiated them from all validly named Acinetobacter species. The inspection of genome sequences in the NCBI database revealed the existence of numerous strains conspecific with this group, which were collected from pig faeces and environmental samples in China. We conclude that the taxon represents an ecologically and geographically widespread species, for which we propose the name Acinetobacter amyesii sp. nov., with ANC 5579T (= CCM 9242T=CCUG 76274T=CNCTC 8134T) as the type strain.
Permanent Link: https://hdl.handle.net/11104/0336514