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Ex-vivo Immune-stimulating Activity of Scutellaria baicalensis and Its Major Flavonoids on Human Immune Cells
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SYSNO ASEP 0556002 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Ex-vivo Immune-stimulating Activity of Scutellaria baicalensis and Its Major Flavonoids on Human Immune Cells Author(s) Vrabec, R. (CZ)
Vokurková, D. (CZ)
Tůmová, L. (CZ)
Cheel Horna, José (MBU-M)Source Title Records of Natural Products. - : ACG Publications - ISSN 1307-6167
Roč. 16, č. 2 (2022), s. 188-193Number of pages 6 s. Language eng - English Country TR - Turkey Keywords activation ; leukocytes ; expression ; baicalin ; antigen ; Scutellaria baicalensis ; immune-stimulation ; cd69 ; flavonoids ; baicalin ; baicalein ; wogonoside Subject RIV EF - Botanics OECD category Plant sciences, botany R&D Projects LO1416 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support MBU-M - RVO:61388971 UT WOS 000690403000008 EID SCOPUS 85114016853 DOI 10.25135/rnp.264.21.02.1976 Annotation This study investigates the activation of human immune cells (T, B, and NK cells, and granulocytes) by the ethanolic and aqueous extracts of Scutellaria baicalensis root (SBR) and to identify the compounds responsible for such an effect. The cell activation was determined by expression of the cell surface glycoprotein CD69 (cluster of differentiation 69), as measured by flow cytometry. The content of the main flavonoids in SBR extracts was estimated by high performance liquid chromatography with a diode array detector. NK cells were substantially activated by the SBR ethanolic extract (25-200 mu g/mL), whereas B cells were activated on a lower scale. NK cells were slightly activated by the SBR aqueous extract (100-200 mu g/mL). Wogonoside and baicalin, two major flavonoids present in the SBR extracts, exhibited the highest activation effect on NK cells. The content of baicalin, wogonoside, and baicalein accounted for 201.2, 47.7, and 3.4 mg/g in the ethanolic extract, and for 150.4, 33.6, and 4.6 mg/g in the aqueous extract, respectively. This study shows the immune-stimulating properties of SBR extracts and identifies their active compounds. This data may support the potential utilization of SBR for strengthening the human immune system against bacterial and viral infections. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2023 Electronic address https://www.acgpubs.org/doc/20210825230226A8-264-RNP-2102-1976.pdf
Number of the records: 1