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Proximity proteomics in a marine diatom reveals a putative cell surface-tochloroplast iron trafficking pathway
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SYSNO ASEP 0555207 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Proximity proteomics in a marine diatom reveals a putative cell surface-tochloroplast iron trafficking pathway Author(s) Turnsek, J. (US)
Brunson, J.K. (US)
Viedma, M. (ES)
Deerinck, T.J. (US)
Horák, Aleš (BC-A) RID, ORCID
Oborník, Miroslav (BC-A) RID, ORCID
Bielinski, V.A. (US)
Allen, A. E. (US)Number of authors 8 Article number e52770 Source Title eLife. - : eLife - ISSN 2050-084X
Roč. 10, FEB 16 2021 (2021)Number of pages 48 s. Publication form Online - E Language eng - English Country GB - United Kingdom Keywords engineered ascorbate peroxidase ; fructose bisphosphate aldolase ; phaeodactylum-tricornutum ; transferrin receptor ; protein-phosphorylation ; functional diversity ; sequence alignment ; fluorescent-probes ; living cells ; h+-atpase Subject RIV EA - Cell Biology OECD category Cell biology R&D Projects GA21-03224S GA ČR - Czech Science Foundation (CSF) EF16_019/0000759 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support BC-A - RVO:60077344 UT WOS 000631066700001 EID SCOPUS 85101941629 DOI 10.7554/eLife.52770 Annotation Iron is a biochemically critical metal cofactor in enzymes involved in photosynthesis, cellular respiration, nitrate assimilation, nitrogen fixation, and reactive oxygen species defense. Marine microeukaryotes have evolved a phytotransferrin-based iron uptake system to cope with iron scarcity, a major factor limiting primary productivity in the global ocean. Diatom phytotransferrin is endocytosed, however, proteins downstream of this environmentally ubiquitous iron receptor are unknown. We applied engineered ascorbate peroxidase APEX2-based subcellular proteomics to catalog proximal proteins of phytotransferrin in the model marine diatom Phaeodactylum tricornutum. Proteins encoded by poorly characterized iron-sensitive genes were identified including three that are expressed from a chromosomal gene cluster. Two of them showed unambiguous colocalization with phytotransferrin adjacent to the chloroplast. Further phylogenetic, domain, and biochemical analyses suggest their involvement in intracellular iron processing. Proximity proteomics holds enormous potential to glean new insights into iron acquisition pathways and beyond in these evolutionarily, ecologically, and biotechnologically important microalgae. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2022 Electronic address https://elifesciences.org/articles/52770
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