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Glyphosate Interaction with eEF1 alpha 1 Indicates Altered Protein Synthesis: Evidence for Reduced Spermatogenesis and Cytostatic Effect
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SYSNO ASEP 0553278 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Glyphosate Interaction with eEF1 alpha 1 Indicates Altered Protein Synthesis: Evidence for Reduced Spermatogenesis and Cytostatic Effect Author(s) Sopko, Bruno (UEM-P) RID, ORCID
Tejral, Gracian (UEM-P)
Bitti, Giuseppe (UEM-P)
Abate, M. (IT)
Medvedíková, M. (CZ)
Hajduch, M. (CZ)
Chloupek, J. (CZ)
Fajmonová, J. (CZ)
Skorič, M. (CZ)
Amler, E. (CZ)
Erban, T. (CZ)Source Title ACS Omega. - : American Chemical Society - ISSN 2470-1343
Roč. 6, č. 23 (2021), s. 14848-14857Number of pages 20 s. Language eng - English Country US - United States Keywords transfer-RNA synthetases ; non-hodgkin-lymphoma ; estrogen-receptor ; cell-growth ; exposure ; expression OECD category Technologies involving identifying the functioning of DNA, proteins and enzymes and how they influence the onset of disease and maintenance of well-being (gene-based diagnostics and therapeutic interventions (pharmacogenomics, gene-based therapeutics) Method of publishing Open access Institutional support UEM-P - RVO:68378041 UT WOS 000663935000012 EID SCOPUS 85108523665 DOI 10.1021/acsomega.1c00449 Annotation The broad-spectrum herbicide, glyphosate, is considered safe for animals because it selectively affects the shikimate pathway that is specific to plants and microorganisms. We sought a previously unknown mechanism to explain the concerns that glyphosate exposure can negatively affect animals, including humans. Computer modeling showed a probable interaction between glyphosate and eukaryotic translation elongation factor 1 subunit alpha 1 (eEF1 alpha 1), which was confirmed by microcalorimetry. Only restricted, nondisrupted spermatogenesis in rats was observed after chronic glyphosate treatments (0.7 and 7 mg/L). Cytostatic and antiproliferative effects of glyphosate in GC-1 and SUP-B15 cells were indicated. Meta-analysis of public health data suggested a possible effect of glyphosate use on sperm count. The in silico, in vitro, and in vivo experimental results as well as the metastatistics indicate side effects of chronic glyphosate exposure. Together, these findings indicate that glyphosate delays protein synthesis through an interaction with eEF1 alpha 1, thereby suppressing spermatogenesis and cell growth. Workplace Institute of Experimental Medicine Contact Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Year of Publishing 2022 Electronic address https://pubs.acs.org/doi/10.1021/acsomega.1c00449
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