Number of the records: 1
Freeze-fracturing of microbes producing biopolymers at liquid Helium temperature: cryo-SEM application in biotechnology
- 1.
SYSNO ASEP 0552527 Document Type A - Abstract R&D Document Type O - Ostatní Title Freeze-fracturing of microbes producing biopolymers at liquid Helium temperature: cryo-SEM application in biotechnology Author(s) Hrubanová, Kamila (UPT-D) RID, SAI, ORCID
Mrázová, Kateřina (UPT-D) SAI, ORCID, RID
Urban, Pavel (UPT-D) RID, ORCID, SAI
Krutil, Vojtěch (UPT-D) ORCID, RID, SAI
Skoupý, Radim (UPT-D) RID, ORCID, SAI
Obruča, S. (CZ)
Krzyžánek, Vladislav (UPT-D) RID, ORCID, SAISource Title Microscopy and Microanalysis. - : Cambridge University Press - ISSN 1431-9276
Roč. 27, S1 (2021), s. 3164-3166Number of pages 3 s. Publication form Online - E Language eng - English Country US - United States Keywords cryo-SEM ; biopolymers ; liquid Helium ; biotechnology Subject RIV JB - Sensors, Measurment, Regulation OECD category Electrical and electronic engineering R&D Projects GA19-20697S GA ČR - Czech Science Foundation (CSF) Institutional support UPT-D - RVO:68081731 DOI 10.1017/S1431927621010941 Annotation Interesting morphologies can be observed when microorganisms containing biopolymeric particles are fractured at very low temperatures far below the glass transition temperatures of this polymer. Some biopolymers such as Polyhydroxyalkanoates (PHAs) can be stretched extensively when fractured at -120°C. This property is different when the same polymer is chemically prepared. In our study was our challenge is to break the sample at an even lower temperature and observe the fracture plane by cryogenic electron microscopy. The new cooling system was developed and applied in several experiments described in this study. Workplace Institute of Scientific Instruments Contact Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178 Year of Publishing 2022 Electronic address https://www.cambridge.org/core/journals/microscopy-and-microanalysis/article/freezefracturing-of-microbes-producing-biopolymers-at-liquid-helium-temperature-cryosem-application-in-biotechnology/50BC0DD9DE37425C157D7C740B5DE39A
Number of the records: 1