Number of the records: 1
Novel technique for definite blastomere inhibition and distribution of maternal RNA in sterlet Acipenser ruthenus embryo
- 1.0550588 - BTÚ 2022 RIV JP eng J - Journal Article
Shah, M. A. - Saito, T. - Šindelka, Radek - Iegorova, Viktoriia - Rodina, M. - Baloch, A. R. - Franěk, R. - Tichopád, T. - Pšenička, M.
Novel technique for definite blastomere inhibition and distribution of maternal RNA in sterlet Acipenser ruthenus embryo.
Fisheries Science. Roč. 87, č. 1 (2021), s. 71-83. ISSN 0919-9268. E-ISSN 1444-2906
R&D Projects: GA ČR(CZ) GA20-23836S
Keywords : uv-b radiation * cytostatic factor * diatoms * gastrulation * evolution
OECD category: Zoology
Impact factor: 2.148, year: 2021
Method of publishing: Limited access
https://link.springer.com/article/10.1007/s12562-020-01481-7
The cleavage pattern of a vertebrate's embryo is either holoblastic (complete) or meroblastic (partial). Sturgeon and other basal bony fishes represent a transition of the cleavage pattern. To understand the transition, it is essential to develop an effective technique for the inhibition of specific blastomere cleavage. So far, various studies have demonstrated that diatom-derived polyunsaturated aldehyde (PUA), 2,4-decadienal (DD)-a model aldehyde for experimental studies-adversely affects the developing embryos of several aquatic species. In this study, we employed DD for inhibition of cleavage of a definite blastomere in sturgeon embryos under various conditions. The effective treatment was found to be a combination of DD injection (0.01 v/v) and visible light (44.86-91.15 W m(-2)). Notably, DD injection or light irradiation alone cannot inhibit cleavage. Furthermore, spatial RNA localization analysis using quantitative polymerase chain reaction (qPCR)-tomography revealed that the localized pattern of selected maternal messenger ribonucleic acids (mRNAs) remained constant along the animal-vegetal (A-V) axis, which suggests that RNA localization is completed by the end of oogenesis and that early embryonic cleavage is not required for A-V asymmetry preservation.
Permanent Link: http://hdl.handle.net/11104/0328022
Number of the records: 1