Number of the records: 1
Rab-dependent vesicular traffic affects female gametophyte development in Arabidopsis
- 1.
SYSNO ASEP 0547985 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Rab-dependent vesicular traffic affects female gametophyte development in Arabidopsis Author(s) Rojek, J. (PL)
Tucker, M. R. (AU)
Pinto, S.C. (BR)
Rychłowski, M. (PL)
Lichocka, M. (PL)
Soukupová, Hana (UEB-Q) RID, ORCID
Nowakowska, J. (PL)
Bohdanowicz, J. (PL)
Surmacz, G. (PL)
Gutkowska, M. (PL)Number of authors 10 Source Title Journal of Experimental Botany. - : Oxford University Press - ISSN 0022-0957
Roč. 72, č. 2 (2021), s. 320-340Number of pages 21 s. Language eng - English Country GB - United Kingdom Keywords Arabidopsis ; auxin transport ; female gametophyte ; funiculus ; ovule ; pin1 ; pin3 ; Rab ; rab geranylgeranyl transferase OECD category Biochemistry and molecular biology Method of publishing Open access Institutional support UEB-Q - RVO:61389030 UT WOS 000648886400014 EID SCOPUS 85111028788 DOI 10.1093/jxb/eraa430 Annotation Eukaryotic cells rely on the accuracy and efficiency of vesicular traffic. In plants, disturbances in vesicular trafficking are well studied in quickly dividing root meristem cells or polar growing root hairs and pollen tubes. The development of the female gametophyte, a unique haploid reproductive structure located in the ovule, has received far less attention in studies of vesicular transport. Key molecules providing the specificity of vesicle formation and its subsequent recognition and fusion with the acceptor membrane are Rab proteins. Rabs are anchored to membranes by covalently linked geranylgeranyl group(s) that are added by the Rab geranylgeranyl transferase (RGT) enzyme. Here we show that Arabidopsis plants carrying mutations in the gene encoding the β-subunit of RGT (rgtb1) exhibit severely disrupted female gametogenesis and this effect is of sporophytic origin. Mutations in rgtb1 lead to internalization of the PIN1 and PIN3 proteins from the basal membranes to vesicles in provascular cells of the funiculus. Decreased transport of auxin out of the ovule is accompanied by auxin accumulation in tissue surrounding the growing gametophyte. In addition, female gametophyte development arrests at the uni- or binuclear stage in a significant portion of the rgtb1 ovules. These observations suggest that communication between the sporophyte and the developing female gametophyte relies on Rab-dependent vesicular traffic of the PIN1 and PIN3 transporters and auxin efflux out of the ovule. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2022 Electronic address http://doi.org/10.1093/jxb/eraa430
Number of the records: 1