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Characterization of Growth and Cell Cycle Events Affected by Light Intensity in the Green Alga Parachlorella kessleri: A New Model for Cell Cycle Research
- 1.0546828 - MBÚ 2022 RIV CH eng J - Journal Article
Zachleder, Vilém - Ivanov, Ivan - Kselíková, Veronika - Bialevich, Vitali - Vítová, Milada - Ota, S. - Takeshita, T. - Kawano, S. - Bišová, Kateřina
Characterization of Growth and Cell Cycle Events Affected by Light Intensity in the Green Alga Parachlorella kessleri: A New Model for Cell Cycle Research.
Biomolecules. Roč. 11, č. 6 (2021), č. článku 891. ISSN 2218-273X. E-ISSN 2218-273X
R&D Projects: GA ČR(CZ) GA17-06264S
Institutional support: RVO:61388971
Keywords : cell cycle pattern * energy reserves * growth processes * light intensity * Parachlorella kessleri * reproduction events * deuterium
OECD category: Biochemistry and molecular biology
Impact factor: 6.064, year: 2021
Method of publishing: Open access
https://www.mdpi.com/2218-273X/11/6/891
Multiple fission is a cell cycle variation leading to the production of more than two daughter cells. Here, we used synchronized cultures of the chlorococcal green alga Parachlorella kessleri to study its growth and pattern of cell division under varying light intensities. The time courses of DNA replication, nuclear and cellular division, cell size, total RNA, protein content, dry matter and accumulation of starch were observed at incident light intensities of 110, 250 and 500 mu mol photons m(-2)s(-1). Furthermore, we studied the effect of deuterated water on Parachlorella kessleri growth and division, to mimic the effect of stress. We describe a novel multiple fission cell cycle pattern characterized by multiple rounds of DNA replication leading to cell polyploidization. Once completed, multiple nuclear divisions were performed with each of them, immediately followed by protoplast fission, terminated by the formation of daughter cells. The multiple fission cell cycle was represented by several consecutive doublings of growth parameters, each leading to the start of a reproductive sequence. The number of growth doublings increased with increasing light intensity and led to division into more daughter cells. This study establishes the baseline for cell cycle research at the molecular level as well as for potential biotechnological applications, particularly directed synthesis of (deuterated) starch and/or neutral lipids as carbon and energy reserves.
Permanent Link: http://hdl.handle.net/11104/0323209
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