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Priming maritime pine megagametophytes during somatic embryogenesis improved plant adaptation to heat stress
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SYSNO ASEP 0546175 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Priming maritime pine megagametophytes during somatic embryogenesis improved plant adaptation to heat stress Author(s) Pérez-Oliver, M. A. (ES)
Haro, J. G. (ES)
Pavlović, Iva (UEB-Q) ORCID
Novák, Ondřej (UEB-Q) RID, ORCID, SAI
Segura, J. (ES)
Sales, E. (ES)
Arrillaga, I. (ES)Number of authors 7 Article number 446 Source Title Plants. - : MDPI
Roč. 10, č. 3 (2021)Number of pages 24 s. Language eng - English Country CH - Switzerland Keywords Heat stress ; Hormones ; hsp ; Photosynthesis ; Pinus pinaster ; Priming ; ros ; Somatic embryogenesis ; Transgenerational memory ; wrky OECD category Plant sciences, botany R&D Projects GA20-22875S GA ČR - Czech Science Foundation (CSF) EF16_019/0000827 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UEB-Q - RVO:61389030 UT WOS 000634069800001 EID SCOPUS 85101632269 DOI 10.3390/plants10030446 Annotation In the context of global climate change, forest tree research should be addressed to provide genotypes with increased resilience to high temperature events. These improved plants can be obtained by heat priming during somatic embryogenesis (SE), which would produce an epigenetic-mediated transgenerational memory. Thereby, we applied 37 °C or 50 °C to maritime pine (Pinus pinaster) megagametophytes and the obtained embryogenic masses went through the subsequent SE phases to produce plants that were further subjected to heat stress conditions. A putative transcription factor WRKY11 was upregulated in priming-derived embryonal masses, and also in the regenerated P37 and P50 plants, suggesting its role in establishing an epigenetic memory in this plant species. In vitro-grown P50 plants also showed higher cytokinin content and SOD upregula-tion, which points to a better responsiveness to heat stress. Heat exposure of two-year-old maritime pine plants induced upregulation of HSP70 in those derived from primed embryogenic masses, that also showed better osmotic adjustment and higher increases in chlorophyll, soluble sugars and starch contents. Moreover, ɸPSII of P50 plants was less affected by heat exposure. Thus, our results suggest that priming at 50 °C at the SE induction phase is a promising strategy to improve heat resilience in maritime pine. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2022 Electronic address http://doi.org/10.3390/plants10030446
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