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Microfluidic on-chip microwave sensing of the self-assembly state of tubulin
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SYSNO ASEP 0543281 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Microfluidic on-chip microwave sensing of the self-assembly state of tubulin Author(s) Krivosudský, Ondrej (URE-Y)
Havelka, Daniel (URE-Y) RID
Chafai, Djamel Eddine (URE-Y)
Cifra, Michal (URE-Y) RID, ORCID, SAINumber of authors 4 Article number 129068 Source Title Sensors and Actuators B - Chemical. - : Elsevier
Roč. 328, FEB 1 2021 (2021)Number of pages 11 s. Publication form Print - P Language eng - English Country CH - Switzerland Keywords Self-assembly ; Proteins ; Tubulin ; Radiofrequency and microwave chips ; High-frequency biosensors Subject RIV JA - Electronics ; Optoelectronics, Electrical Engineering OECD category Electrical and electronic engineering R&D Projects GX20-06873X GA ČR - Czech Science Foundation (CSF) Method of publishing Limited access Institutional support URE-Y - RVO:67985882 UT WOS 000600962800008 DOI 10.1016/j.snb.2020.129068 Annotation Self-assembly is at the heart of many promising nanoscience technologies as well as at the core of life processes. Tubulin proteins self-assemble into microtubules, tube-like structures that are essential in cellular functions such as cell division and intracellular transport and also a major target in cancer therapies. Therefore, it is crucial to develop efficient methods for monitoring tubulin self-assembly at the molecular level. To mitigate the limitations of current methods, we propose a new approach based on a microwave lab-on-a-chip method to monitor tubulin self-assembly states. To that end, we designed a dedicated microwave platform with integrated microfluidics with a sensing volume of < 30 nL. In parallel, we used a standard bulk light-scattering-based method to assess the tubulin self-assembly and to validate the microwave chip results. Using our chip, for the first time, we demonstrate that the self-assembly state of tubulin into microtubules can be monitored using microwave microfluidics technology. Our results introduce a novel label-free electromagnetic monitoring and analytical method for bionanotechnology and biomedicine applications that can potentially be integrated into advanced microscopy systems Workplace Institute of Radio Engineering and Electronics Contact Petr Vacek, vacek@ufe.cz, Tel.: 266 773 413, 266 773 438, 266 773 488 Year of Publishing 2022 Electronic address https://doi.org/10.1016/j.snb.2020.129068
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