Cytokinin n-glucosides: Occurrence, metabolism and biological activities in plants

Pokorná, Eva; Hluska, Tomáš; Galuszka, P.; Hallmark, H. T.; Dobrev, Petre; Záveská Drábková, Lenka; Filipi, T.; Holubová, K.; Plíhal, Ondřej; Rashotte, A.M.; Filepová, Roberta; Malbeck, Jiří; Novák, Ondřej; Spíchal, L.; Brzobohatý, Břetislav; Mazura, P.; Zahajská, Lenka; Motyka, Václav

doi:https://dx.doi.org/10.3390/biom11010024

Number of the records: 1

Cytokinin n-glucosides: Occurrence, metabolism and biological activities in plants

1.

SYSNO ASEP	0542844
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Cytokinin n-glucosides: Occurrence, metabolism and biological activities in plants
Author(s)	Pokorná, Eva (UEB-Q)_ORCID Hluska, Tomáš (UEB-Q)_ORCID Galuszka, P. (CZ) Hallmark, H. T. (US) Dobrev, Petre (UEB-Q)_{RID, ORCID} Záveská Drábková, Lenka (UEB-Q)_ORCID Filipi, T. (CZ) Holubová, K. (CZ) Plíhal, Ondřej (UEB-Q)_ORCID Rashotte, A.M. (US) Filepová, Roberta (UEB-Q)_SAI Malbeck, Jiří (UEB-Q)_{RID, ORCID} Novák, Ondřej (UEB-Q)_{RID, ORCID, SAI} Spíchal, L. (CZ) Brzobohatý, Břetislav (BFU-R)_{RID, ORCID} Mazura, P. (CZ) Zahajská, Lenka (UEB-Q)_{RID, ORCID} Motyka, Václav (UEB-Q)_{RID, ORCID}
Number of authors	18
Article number	24
Source Title	Biomolecules. - : MDPI Roč. 11, č. 1 (2021)
Number of pages	28 s.
Language	eng - English
Country	CH - Switzerland
Keywords	Arabidopsis ; Cis-zeatin ; Cytokinin ; Cytokinin oxidase/dehydrogenase ; N-glucosides ; Oat ; Senescence ; Trans-zeatin ; Zea mays ; [2-3H]tZ9G 3 ; β-D-glucosidase Zm-p60.1
Subject RIV	EF - Botanics
OECD category	Plant sciences, botany
R&D Projects	GA19-12262S GA ČR - Czech Science Foundation (CSF)
	GA19-13103S GA ČR - Czech Science Foundation (CSF)
	GA19-02699S GA ČR - Czech Science Foundation (CSF)
	EF16_019/0000738 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	LQ1601 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Method of publishing	Open access
Institutional support	UEB-Q - RVO:61389030 ; BFU-R - RVO:68081707
UT WOS	000609847800001
EID SCOPUS	85098587182
DOI	10.3390/biom11010024
Annotation	Cytokinins (CKs) are a class of phytohormones affecting many aspects of plant growth and development. In the complex process of CK homeostasis in plants, N-glucosylation represents one of the essential metabolic pathways. Its products, CK N7-and N9-glucosides, have been largely overlooked in the past as irreversible and inactive CK products lacking any relevant physiological impact. In this work, we report a widespread distribution of CK N-glucosides across the plant kingdom proceeding from evolutionary older to younger plants with different proportions between N7-and N9-glucosides in the total CK pool. We show dramatic changes in their profiles as well as in expression levels of the UGT76C1 and UGT76C2 genes during Arabidopsis ontogenesis. We also demonstrate specific physiological effects of CK N-glucosides in CK bioassays including their antisenescent activities, inhibitory effects on root development, and activation of the CK signaling pathway visualized by the CK-responsive YFP reporter line, TCSv2::3XVENUS. Last but not least, we present the considerable impact of CK N7-and N9-glucosides on the expression of CK-related genes in maize and their stimulatory effects on CK oxidase/dehydrogenase activity in oats. Our findings revise the apparent irreversibility and inactivity of CK N7-and N9-glucosides and indicate their involvement in CK evolution while suggesting their unique function(s) in plants.
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2022
Electronic address	http://doi.org/10.3390/biom11010024

Number of the records: 1