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Laser-induced breakdown spectroscopy as a straightforward bioimaging tool for plant biologists, the case study for assessment of photon-upconversion nanoparticles in Brassica oleracea L. plant
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SYSNO ASEP 0541897 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Laser-induced breakdown spectroscopy as a straightforward bioimaging tool for plant biologists, the case study for assessment of photon-upconversion nanoparticles in Brassica oleracea L. plant Author(s) Modlitbová, P. (CZ)
Střítežská, Š. (CZ)
Hlaváček, Antonín (UIACH-O) ORCID
Procházka, D. (CZ)
Pořízka, P. (CZ)
Kaiser, J. (CZ)Number of authors 6 Article number 112113 Source Title Ecotoxicology and Enviromental Safety. - : Elsevier - ISSN 0147-6513
Roč. 214, MAY (2021)Number of pages 9 s. Publication form Print - P Language eng - English Country US - United States Keywords rare-earth elements ; spatial element distribution ; translocation ; bioaccumulation Subject RIV CB - Analytical Chemistry, Separation OECD category Analytical chemistry R&D Projects GJ18-03367Y GA ČR - Czech Science Foundation (CSF) Method of publishing Limited access Institutional support UIACH-O - RVO:68081715 UT WOS 000634521900006 EID SCOPUS 85102044842 DOI 10.1016/j.ecoenv.2021.112113 Annotation The main purpose of this work is to thoroughly describe the implementation protocol of laser-induced breakdown spectroscopy (LIBS) method in the plant analysis. Numerous feasibility studies and recent progress in instrumentation and trends in chemical analysis make LIBS an established method in plant bioimaging. In this work, we present an easy and straightforward phytotoxicity case study with a focus on LIBS method. We intend to demonstrate in detail how to manipulate with plants after exposures and how to prepare them for analyses. Moreover, we aim to achieve 2D maps of spatial element distribution with a good resolution without any loss of sensitivity. The benefits of rapid, low-cost bioimaging are highlighted. Workplace Institute of Analytical Chemistry Contact Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Year of Publishing 2022 Electronic address http://hdl.handle.net/11104/0319393
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