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Accelerated in vitro recellularization of decellularized porcine pericardium for cardiovascular grafts
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SYSNO ASEP 0541894 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Accelerated in vitro recellularization of decellularized porcine pericardium for cardiovascular grafts Author(s) Filová, Elena (FGU-C) RID, ORCID
Steinerová, Marie (FGU-C) ORCID
Trávníčková, Martina (FGU-C) RID, ORCID, SAI
Knitlová, Jarmila (FGU-C) RID, ORCID
Musílková, Jana (FGU-C) RID, ORCID
Eckhardt, Adam (FGU-C) RID, ORCID
Hadraba, Daniel (FGU-C) RID, ORCID, SAI
Matějka, Roman (FGU-C) RID, ORCID, SAI
Pražák, Šimon (FGU-C) ORCID
Štěpanovská, Jana (FGU-C) ORCID, RID
Kučerová, Johanka (UMCH-V)
Riedel, Tomáš (UMCH-V) RID, ORCID
Brynda, Eduard (UMCH-V) RID
Lodererová, A. (CZ)
Honsová, E. (CZ)
Pirk, J. (CZ)
Koňařík, M. (CZ)
Bačáková, Lucie (FGU-C) RID, ORCIDArticle number 025024 Source Title Biomedical Materials. - : Institute of Physics Publishing - ISSN 1748-6041
Roč. 16, č. 2 (2021)Number of pages 13 s. Language eng - English Country GB - United Kingdom Keywords adipose tissue-derived stem cells ; repopulation ; decellularized pericardium ; human umbilical cells ; modification with fibrin ; heparin ; vascular endothelial growth factor Subject RIV FA - Cardiovascular Diseases incl. Cardiotharic Surgery OECD category Cardiac and Cardiovascular systems Subject RIV - cooperation Institute of Macromolecular Chemistry - Macromolecular Chemistry R&D Projects NV15-29153A GA MZd - Ministry of Health (MZ) NV18-02-00422 GA MZd - Ministry of Health (MZ) LQ1604 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LM2015062 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Research Infrastructure Czech-BioImaging - 90062 - Ústav molekulární genetiky AV ČR, v. v. i. Method of publishing Limited access Institutional support FGU-C - RVO:67985823 ; UMCH-V - RVO:61389013 UT WOS 000621486800001 EID SCOPUS 85100632730 DOI 10.1088/1748-605X/abbdbd Annotation An ideal decellularized allogenic or xenogeneic cardiovascular graft should be capable of preventing thrombus formation after implantation. The antithrombogenicity of the graft is ensured by a confluent endothelial cell layer formed on its surface. Later repopulation and remodeling of the scaffold by the patient's cells should result in the formation of living autologous tissue. In the work presented here, decellularized porcine pericardium scaffolds were modified by growing a fibrin mesh on the surface and inside the scaffolds, and by attaching heparin and human vascular endothelial growth factor (VEGF) to this mesh. Then the scaffolds were seeded with human adipose tissue-derived stem cells (ASCs). While the ASCs grew only on the surface of the decellularized pericardium, the fibrin-modified scaffolds were entirely repopulated in 28 d, and the scaffolds modified with fibrin, heparin and VEGF were already repopulated within 6 d. Label free mass spectrometry revealed fibronectin, collagens, and other extracellular matrix proteins produced by ASCs during recellularization. Thin layers of human umbilical endothelial cells were formed within 4 d after the cells were seeded on the surfaces of the scaffold, which had previously been seeded with ASCs. The results indicate that an artificial tissue prepared by in vitro recellularization and remodeling of decellularized non-autologous pericardium with autologous ASCs seems to be a promising candidate for cardiovascular grafts capable of accelerating in situ endothelialization. ASCs resemble the valve interstitial cells present in heart valves. An advantage of this approach is that ASCs can easily be collected from the patient by liposuction. Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2022 Electronic address https://iopscience.iop.org/article/10.1088/1748-605X/abbdbd
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