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DNA replication and chromosome positioning throughout the interphase in three-dimensional space of plant nuclei
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SYSNO ASEP 0538924 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title DNA replication and chromosome positioning throughout the interphase in three-dimensional space of plant nuclei Author(s) Němečková, Alžběta (UEB-Q) ORCID
Koláčková, Veronika (UEB-Q) ORCID
Vrána, Jan (UEB-Q) RID, ORCID
Doležel, Jaroslav (UEB-Q) RID, ORCID
Hřibová, Eva (UEB-Q) RID, ORCIDNumber of authors 5 Source Title Journal of Experimental Botany. - : Oxford University Press - ISSN 0022-0957
Roč. 71, č. 20 (2020), s. 6262-6272Number of pages 11 s. Language eng - English Country GB - United Kingdom Keywords genome-wide analysis ; s-phase ; arabidopsis-thaliana ; chromatin packing ; timing program ; allium-cepa ; organization ; telomeres ; domains ; dynamics ; DNA replication ; EdU labeling ; flow cytometry ; Poaceae ; Rabl configuration ; S phase ; three-dimensional fluorescence in situ hybridization (3D-FISH) Subject RIV EB - Genetics ; Molecular Biology OECD category Biochemistry and molecular biology R&D Projects LM2015062 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA17-14048S GA ČR - Czech Science Foundation (CSF) EF16_019/0000827 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UEB-Q - RVO:61389030 UT WOS 000605983900008 EID SCOPUS 85095718378 DOI 10.1093/jxb/eraa370 Annotation Despite much recent progress, our understanding of the principles of plant genome organization and its dynamics in three-dimensional space of interphase nuclei remains surprisingly limited. Notably, it is not clear how these processes could be affected by the size of a plant's nuclear genome. In this study, DNA replication timing and interphase chromosome positioning were analyzed in seven Poaceae species that differ in their genome size. To provide a comprehensive picture, a suite of advanced, complementary methods was used: labeling of newly replicated DNA by ethynyl-2'-deoxyuridine, isolation of nuclei at particular cell cycle phases by flow cytometric sorting, three-dimensional immunofluorescence in situ hybridization, and confocal microscopy. Our results revealed conserved dynamics of DNA replication in all species, and a similar replication timing order for telomeres and centromeres, as well as for euchromatin and heterochromatin regions, irrespective of genome size. Moreover, stable chromosome positioning was observed while transitioning through different stages of interphase. These findings expand upon earlier studies in suggesting that a more complex interplay exists between genome size, organization of repetitive DNA sequences along chromosomes, and higher order chromatin structure and its maintenance in interphase, albeit controlled by currently unknown factors. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2021 Electronic address http://doi.org/10.1093/jxb/eraa370
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