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Large volume sample stacking of antiepileptic drugs in counter current electrophoresis performed in PAMAPTAC coated capillary
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SYSNO ASEP 0536465 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Large volume sample stacking of antiepileptic drugs in counter current electrophoresis performed in PAMAPTAC coated capillary Author(s) Tůma, P. (CZ)
Hložek, T. (CZ)
Sommerová, B. (CZ)
Koval, Dušan (UOCHB-X) RID, ORCIDArticle number 121626 Source Title Talanta. - : Elsevier - ISSN 0039-9140
Roč. 221, Jan 1 (2021)Number of pages 8 s. Language eng - English Country NL - Netherlands Keywords antiepileptic ; capillary electrophoresis ; coating ; contactless conductivity detection ; sample pretreatment ; serum ; stacking OECD category Analytical chemistry Method of publishing Limited access Institutional support UOCHB-X - RVO:61388963 UT WOS 000579910600109 EID SCOPUS 85090586050 DOI 10.1016/j.talanta.2020.121626 Annotation Electrophoretic stacking is developed for sensitive determination of three zwitterionic antiepileptics, namely vigabatrin, pregabalin and gabapentin, in human serum. CE separation is performed in a 25 μm fused silica capillary covalently coated with the copolymer of acrylamide with 5% content of permanently charged 3-acrylamidopropyl trimethylammonium chloride (PAMAPTAC). In background electrolyte of 500 mM acetic acid, the 5% PAMAPTAC generates an anodic electro-osmotic flow with a magnitude of (−18.6 ± 0.5) · 10−9 m2V−1s−1, which acts against the direction of the electrophoretic migration of the analytes. A sample of the antiepileptic prepared in a 25% v/v infusion solution and 75% v/v acetonitrile is injected into the capillary in a large volume attaining a zone length of up to 270 mm. After turning on the separation voltage, the antiepileptics are isotachophoretically focussed behind the zone of Na+ ions with a sensitivity enhancement factor of 78. For the clinical determination of antiepileptics, the human serum is diluted with acetonitrile in a ratio of 1:3 v/v and a zone with a length of 90 mm is injected into the capillary. The method is linear in the 0.025–2.5 μg/mL concentration range, the attained limit of quantification is in the range 18.3–22.8 nmol/L, the within-day precision for the migration time is 0.8–1.2% and for the peak area 1.5–2.4%. Workplace Institute of Organic Chemistry and Biochemistry Contact asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418 Year of Publishing 2022 Electronic address https://doi.org/10.1016/j.talanta.2020.121626
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