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Cytogenetic Characterization of Seven Novel satDNA Markers in Two Species of Spined Loaches (Cobitis) and Their Clonal Hybrids
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SYSNO ASEP 0531710 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Cytogenetic Characterization of Seven Novel satDNA Markers in Two Species of Spined Loaches (Cobitis) and Their Clonal Hybrids Author(s) Marta, Anatolie (UZFG-Y) ORCID
Didukh, Dmitrij (UZFG-Y) ORCID
Bartoš, Oldřich (UZFG-Y)
Majtánová, Zuzana (UZFG-Y) RID, ORCID
Janko, Karel (UZFG-Y) RID, ORCIDArticle number 647 Source Title Genes. - : MDPI
Roč. 11, č. 6 (2020)Number of pages 16 s. Publication form Online - E Language eng - English Country CH - Switzerland Keywords clonal vertebrates ; hybridization ; satellite DNA ; FISH ; mitotic and lampbrush chromosomes Subject RIV EB - Genetics ; Molecular Biology OECD category Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology R&D Projects GA19-21552S GA ČR - Czech Science Foundation (CSF) EF15_003/0000460 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UZFG-Y - RVO:67985904 UT WOS 000550919700001 EID SCOPUS 85086168899 DOI 10.3390/genes11060617 Annotation Interspecific hybridization is a powerful evolutionary force. However, the investigation of hybrids requires the application of methodologies that provide efficient and indubitable identification of both parental subgenomes in hybrid individuals. Repetitive DNA, and especially the satellite DNA sequences (satDNA), can rapidly diverge even between closely related species, hence providing a useful tool for cytogenetic investigations of hybrids. Recent progress in whole-genome sequencing (WGS) offers unprecedented possibilities for the development of new tools for species determination, including identification of species-specific satDNA markers. In this study, we focused on spined loaches (Cobitis, Teleostei), a group of fishes with frequent interspecific hybridization. Using the WGS of one species,C. elongatoides, we identified seven satDNA markers, which were mapped by fluorescence in situ hybridization on mitotic and lampbrush chromosomes ofC. elongatoides,C. taeniaand their triploid hybrids (C. elongatoidesx 2C. taenia). Two of these markers were chromosome-specific in both species, one had centromeric localization in multiple chromosomes and four had variable patterns between tested species. Our study provided a novel set of cytogenetic markers forCobitisspecies and demonstrated that NGS-based development of satDNA cytogenetic markers may provide a very efficient and easy tool for the investigation of hybrid genomes, cell ploidy, and karyotype evolution. Workplace Institute of Animal Physiology and Genetics Contact Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Year of Publishing 2021 Electronic address https://www.mdpi.com/2073-4425/11/6/617
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