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PRP8A and PRP8B spliceosome subunits act coordinately to control pollen tube attraction in Arabidopsis thaliana
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SYSNO ASEP 0531677 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title PRP8A and PRP8B spliceosome subunits act coordinately to control pollen tube attraction in Arabidopsis thaliana Author(s) Kulichová, Katarína (UEB-Q) ORCID
Kumar, Vinod (UEB-Q) ORCID
Steinbachová, Lenka (UEB-Q) ORCID, RID
Klodová, Božena (UEB-Q) ORCID
Timofejeva, Ljudmilla (UEB-Q) ORCID
Juříček, Miloslav (UEB-Q) RID, ORCID
Honys, David (UEB-Q) RID, ORCID
Hafidh, Said (UEB-Q) RID, ORCIDNumber of authors 8 Article number dev186742 Source Title Development. - : Company of Biologists - ISSN 0950-1991
Roč. 147, č. 11 (2020)Number of pages 18 s. Language eng - English Country GB - United Kingdom Keywords Cell-to-cell signaling ; Cysteine-rich proteins ; Pollen tube attraction and reception ; Post-transcriptional regulation ; Pre-mRNA PROCESSING factor 8 ; Sexual reproduction ; Splicing Subject RIV EF - Botanics OECD category Plant sciences, botany R&D Projects GA17-23203S GA ČR - Czech Science Foundation (CSF) GA18-02448S GA ČR - Czech Science Foundation (CSF) GA19-01723S GA ČR - Czech Science Foundation (CSF) LTAUSA18115 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) EF16_019/0000738 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LM2018129 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UEB-Q - RVO:61389030 UT WOS 000541761600009 EID SCOPUS 85086525338 DOI 10.1242/dev.186742 Annotation Precise guided pollen tube growth by the female gametophyte is a prerequisite for successful sexual reproduction in flowering plants. Cysteine-rich proteins (CRPs) secreted from the embryo sac are known pollen tube attractants perceived by pollen tube receptor-like kinases. How pre-mRNA splicing facilitates this cell-to-cell communication is not understood. Here, we report a novel function of Pre-mRNA PROCESSING factor 8 paralogs, PRP8A and PRP8B, as regulators of pollen tube attraction. Double mutant prp8a prp8b ovules cannot attract pollen tubes, and prp8a prp8b pollen tubes fail to sense the ovule's attraction signals. Only 3% of ovule-expressed genes were misregulated in prp8a prp8b Combination of RNA sequencing and the MYB98/LURE1.2-YFP reporter revealed that the expression of MYB98, LUREs and 49 other CRPs were downregulated, suggesting loss of synergid cell fate. Differential exon usage and intron retention analysis revealed autoregulation of PPR8A/PRP8B splicing. In vivo, PRP8A co-immunoprecipitates with splicing enhancer AtSF3A1, suggesting involvement of PRP8A in 3'-splice site selection. Our data hint that the PRP8A/PRP8B module exhibits spliceosome autoregulation to facilitate pollen tube attraction via transcriptional regulation of MYB98, CRPs and LURE pollen tube attractants. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2021 Electronic address http://doi.org/10.1242/dev.186742
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