Characterization and in vitro assembly of tick-borne encephalitis virus C protein

0531460 - ÚOCHB 2021 RIV NL eng J - Journal Article
Kaufman, F. - Dostálková, A. - Pekárek, L. - Thanh, T. D. - Kapisheva, M. - Hadravová, Romana - Bednárová, Lucie - Novotný, R. - Křížová, I. - Černý, J. - Grubhoffer, Libor - Ruml, T. - Hrabal, R. - Rumlová, M.
Characterization and in vitro assembly of tick-borne encephalitis virus C protein.
FEBS Letters. Roč. 594, č. 12 (2020), s. 1989-2004. ISSN 0014-5793. E-ISSN 1873-3468
R&D Projects: GA ČR(CZ) GA18-27204S
Institutional support: RVO:61388963 ; RVO:60077344
Keywords : assembly * C protein * DENV * flavivirus * protein-nucleic acid interaction * TBEV
OECD category: Biochemistry and molecular biology; Virology (BC-A)
Impact factor: 4.124, year: 2020
Method of publishing: Limited access
https://doi.org/10.1002/1873-3468.13857

Tick‐borne encephalitis virus (TBEV), a member of flaviviruses, represents a serious health threat by causing human encephalitis mainly in central and eastern Europe, Russia, and northeastern Asia. As no specific therapy is available, there is an urgent need to understand all steps of the TBEV replication cycle at the molecular level. One of the critical events is the packaging of flaviviral genomic RNA by TBEV C protein to form a nucleocapsid. We purified recombinant TBEV C protein and used a combination of physical–chemical approaches, such as size‐exclusion chromatography, circular dichroism, NMR spectroscopies, and transmission electron microscopy, to analyze its structural stability and its ability to dimerize/oligomerize. We compared the ability of TBEV C protein to assemble in vitro into a nucleocapsid‐like structure with that of dengue C protein.
Permanent Link: http://hdl.handle.net/11104/0310121