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Proteomic analysis of the cardiac myocyte secretome reveals extracellular protective functions for the ER stress response
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SYSNO ASEP 0524331 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Proteomic analysis of the cardiac myocyte secretome reveals extracellular protective functions for the ER stress response Author(s) Blackwood, E. A. (US)
Thuerauf, D. J. (US)
Šťastná, Miroslava (UIACH-O) RID, ORCID
Stephens, H. (US)
Sand, Z. (US)
Pentoney, A. (US)
Azizi, K. (US)
Jakobi, T. (DE)
Van Eyk, J.E. (US)
Katus, H. A. (DE)
Glembotski, Ch. C. (US)
Doroudgar, S. (DE)Number of authors 12 Source Title Journal of Molecular and Cellular Cardiology. - : Elsevier - ISSN 0022-2828
Roč. 143, JUN (2020), s. 132-144Number of pages 13 s. Publication form Print - P Language eng - English Country GB - United Kingdom Keywords ER stress ; cardioprotection ; secreted proteins Subject RIV CB - Analytical Chemistry, Separation OECD category Analytical chemistry Method of publishing Limited access Institutional support UIACH-O - RVO:68081715 UT WOS 000579927300012 EID SCOPUS 85084362964 DOI 10.1016/j.yjmcc.2020.04.012 Annotation The effect of ER stress on protein secretion by cardiac myocytes using ER stressors thapsigargin (TG), which depletes ER calcium, and tunicamycin (TM) was studied. Proteomic analyses of TG- and TM-conditioned media showed that the secretion of most proteins was inhibited by TG and TM, however, secretion of several ER-resident proteins, including GRP78 was increased by TG but not TM. Simulated ischemia, which decreases SR/ER calcium also increased secretion of these proteins. The proteins secreted during ER stress mediated by ER calcium depletion can enhance cardiac myocyte viability. Workplace Institute of Analytical Chemistry Contact Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Year of Publishing 2021 Electronic address http://hdl.handle.net/11104/0308692
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